ydhE

168

similar to macrolide glycosyltransferase

locus: BSU_05720

Molecular weight: 42.87 kDa

pI: 5.09

Protein length: 395 aa

Gene length: 1188 bp

function: unknown

product: unknown

essential: no

synonyms: ydhE

List of homologs in different organisms, belongs to COG1819 (Galperin et al., 2021)

Gene

Coordinates: 618,095 → 619,282

The protein

Protein family

• UDP-glycosyltransferase family (with [protein|02927D2CC9F44B99DB307DDFED2DD52DE2196120|yjiC] and [protein|6D808BC20ADB6CA789288D50CA816D6BEE22CFC3|yojK], according to UniProt)

Structure

• [PDB|2IYA] (from Streptomyces antibioticus, 28% identity) [pubmed|17376874]
• [AF|O05496]

Expression and Regulation

Operons

• genes: [gene|BD82663B1BF2763D2D2DE710C2896E4155419324|ydhE]

  description: [Pubmed|20639339]

  regulatory mechanism

  • [protein|49E70A20CC05DBE485953C0AE34E634EFB33C3B2|liaR]: activation, weak, in [regulon|protein:49E70A20CC05DBE485953C0AE34E634EFB33C3B2|liaR regulon]

Biological materials

Mutant

• MGNA-C180 (ydhE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2178 NBRP B. subtilis, Japan]
• BKE05720 (Δ[gene|BD82663B1BF2763D2D2DE710C2896E4155419324|ydhE]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE05720 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTAGACAACTCCCTCTC,  downstream forward: _UP4_TAAGAAAAAGAACTCCCGTA
• BKK05720 (Δ[gene|BD82663B1BF2763D2D2DE710C2896E4155419324|ydhE]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK05720 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTAGACAACTCCCTCTC,  downstream forward: _UP4_TAAGAAAAAGAACTCCCGTA

References

• 20639339,27130432,17376874,31786106