mneP

168

primary manganese (II) efflux pump, contributes to iron efflux

locus: BSU_05470

Molecular weight: 32.64 kDa

pI: 6.16

Protein length: 297 aa

Gene length: 894 bp

function: Mn(II) export

product: manganese (II) efflux pump

essential: no

synonyms: mneP, ydfM

List of homologs in different organisms, belongs to COG0053 (Galperin et al., 2021)

Gene

Coordinates: 595,109  596,002

Phenotypes of a mutant

• sensitive to Mn(II) [Pubmed|27748968]
• a [gene|28C7CFE5701F6920652BE1973DC7A15D7C088E35|mneP] [gene|72BF9B934CB5800E156263FF377DED0AD2E920A9|mneS] double mutant is extremely senitive to Mn²⁺ intoxication, this results in generation of reactive radicals by poisoned cytochrome aa₃ oxidase [protein|48A9E1E39838BFFCF12CADF0A8D8E6FFDCA6F175|qoxA]-[protein|CBF2DCFFD8195304F942ECBA8596444F0AC47CC6|qoxB]-[protein|CD4520EF427AEFDCF028AE063BC373FA6DCE379E|qoxC]-[protein|03335DC50CED7B38E2380640AE108ADA09A2CBDF|qoxD] and can be suppressed by inactivation of [protein|48A9E1E39838BFFCF12CADF0A8D8E6FFDCA6F175|qoxA]-[protein|CBF2DCFFD8195304F942ECBA8596444F0AC47CC6|qoxB]-[protein|CD4520EF427AEFDCF028AE063BC373FA6DCE379E|qoxC]-[protein|03335DC50CED7B38E2380640AE108ADA09A2CBDF|qoxD] (any gene) or of [gene|997B828A99D6D8460712C18ED0CE566B285C22DC|mhqR] to activate the electrophile stress response [pubmed|34097790,31685536]

The protein

Catalyzed reaction/ biological activity

• efflux of Mn(II) [Pubmed|27748968]
• efflux of iron [pubmed|36748638]

Protein family

• [wiki|Cation diffusion facilitator (CDF) transporter (TC 2.A.4) family] [Pubmed|27748968]

Structure

• [PDB|5VRF] (Shewanella oneidensis zinc transporter, 26% identity) [pubmed|29507252]
• [AF|C0SP78]

Paralogous protein(s)

• [protein|72BF9B934CB5800E156263FF377DED0AD2E920A9|mneS], [protein|D52111E81E9233DABAEFCAF6217145CF6A7F3961|ydbO]

[wiki|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|28C7CFE5701F6920652BE1973DC7A15D7C088E35|mneP]

  description: [pubmed|22383849]

  regulation

  • expressed at elevated Mn(II) concentrations ([protein|FF34DC303E8A7C9FAA205A6C2924961963B14EB1|mntR]) [Pubmed|27748968]

  regulatory mechanism

  • [protein|FF34DC303E8A7C9FAA205A6C2924961963B14EB1|mntR]: transcription activation, [Pubmed|27748968], in [regulon|protein:FF34DC303E8A7C9FAA205A6C2924961963B14EB1|mntR regulon]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|27748968], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

Biological materials

Mutant

• MGNA-C152 (ydfM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2150 NBRP B. subtilis, Japan]
• BKE05470 ([gene|28C7CFE5701F6920652BE1973DC7A15D7C088E35|mneP]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE05470 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTTTTCCTCCTATAAAA,  downstream forward: _UP4_TGAAAAGCTCCTTTTTAGAA
• BKK05470 ([gene|28C7CFE5701F6920652BE1973DC7A15D7C088E35|mneP]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK05470 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTTTTCCTCCTATAAAA,  downstream forward: _UP4_TGAAAAGCTCCTTTTTAGAA

References

Reviews

• 34037759

Original Publications

• 27748968,29507252,31685536,34097790,36748638,37468100,38819154,38979284