mneP

168

primary manganese (II) efflux pump, contributes to iron efflux

Locus: BSU_05470

Molecular weight: 32.64 kDa

Isoelectric point: 6.16

Protein length: 297 aa

Gene length: 894 bp

Function: Mn(II) export

Product: manganese (II) efflux pump

Essential: no

Synonyms: mneP, ydfM

Genomic Context

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List of homologs in different organisms, belongs to COG0053 (Galperin et al., 2021)

Gene

Coordinates: 595,109  596,002

Phenotypes of a mutant

• sensitive to Mn(II) PubMed
• a mneP mneS double mutant is extremely senitive to Mn²⁺ intoxication, this results in generation of reactive radicals by poisoned cytochrome aa₃ oxidase QoxA-QoxB-QoxC-QoxD and can be suppressed by inactivation of QoxA-QoxB-QoxC-QoxD (any gene) or of mhqR to activate the electrophile stress response PubMed

The protein

Catalyzed reaction/ biological activity

• efflux of Mn(II) PubMed
• efflux of iron PubMed

Protein family

• Cation diffusion facilitator (CDF) transporter (TC 2.A.4) family PubMed

Structure

• 5VRF (PDB) (Shewanella oneidensis zinc transporter, 26% identity) PubMed
• C0SP78 (AlphaFold)

Paralogous protein(s)

• MneS, YdbO

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: mneP

  Description: PubMed

  Regulation

  • expressed at elevated Mn(II) concentrations (MntR) PubMed

  Regulatory mechanism

  • MntR: transcription activation, PubMed, in mntR regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• MGNA-C152 (ydfM::erm), available at the NBRP B. subtilis, Japan
• BKE05470 (mneP::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTTTTCCTCCTATAAAA,  downstream forward: _UP4_TGAAAAGCTCCTTTTTAGAA
• BKK05470 (mneP::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTTTTCCTCCTATAAAA,  downstream forward: _UP4_TGAAAAGCTCCTTTTTAGAA

References

Reviews

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Original Publications

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