recF

168

promoter of RecA DNA repair center assembly

Locus: BSU_00040

Molecular weight: 42.15 kDa

Isoelectric point: 7.01

Protein length: 370 aa

Gene length: 1113 bp

Function: DNA repair/ recombination

Product: promoter of RecA DNA repair center assembly

Synonyms: recF

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG1195 (Galperin et al., 2021)

Gene

Coordinates: 3,437 → 4,549

Phenotypes of a mutant

• the mutant tends to acquire suppressor mutations that result in improved growth PubMed
• drastically reduced survival of mature dormant spores after exposure to ultrahigh vacuum desiccation and ionizing radiation that induce single strand (ss) DNA nicks and double-strand breaks (DSBs) PubMed

The protein

Catalyzed reaction/ biological activity

• increases the efficiency of RecA DNA repair center assembly PubMed

Protein family

• RecF family (single member, according to UniProt)

Structure

• 5Z67 (PDB) (from Thermoanaerobacter tengcongensis, 33% identity) PubMed
• P05651 (AlphaFold)

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: yaaA-recF-remB-gyrB-gyrA

  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• GP3529 (ΔrecF::spec), available in Jörg Stülke's lab
• BKE00040 (ΔrecF::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GATATACAATCAGTGTCACC,  downstream forward: _UP4_AAGTGAAGAAATGAGGTGAG
• BKK00040 (ΔrecF::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GATATACAATCAGTGTCACC,  downstream forward: _UP4_AAGTGAAGAAATGAGGTGAG

References

Reviews

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Original Publications

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