Difference between revisions of "MccB"
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|style="background:#ABCDEF;" align="center"|'''Function''' || methionine-to-cysteine conversion | |style="background:#ABCDEF;" align="center"|'''Function''' || methionine-to-cysteine conversion | ||
|- | |- | ||
| − | |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/ | + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU27250 mccB] |
| + | |- | ||
| + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=mccB mccB]''' | ||
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 40 kDa, 5.215 | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 40 kDa, 5.215 | ||
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yrhC]]'', ''[[mccA]]'' | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yrhC]]'', ''[[mccA]]'' | ||
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| − | | | + | |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU27250 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU27250 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU27250 DNA_with_flanks] |
|- | |- | ||
|colspan="2" | '''Genetic context''' <br/> [[Image:yrhB_context.gif]] | |colspan="2" | '''Genetic context''' <br/> [[Image:yrhB_context.gif]] | ||
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div> | <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div> | ||
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| − | |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=mccB_2785001_2786140_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:mccB_expression.png|500px]] | + | |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=mccB_2785001_2786140_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:mccB_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU27250]] |
|- | |- | ||
|} | |} | ||
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=== Database entries === | === Database entries === | ||
| + | * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU27250&redirect=T BSU27250] | ||
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yrrT-mtn-yrhABC.html] | * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yrrT-mtn-yrhABC.html] | ||
| Line 96: | Line 99: | ||
=== Database entries === | === Database entries === | ||
| + | * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU27250&redirect=T BSU27250] | ||
* '''Structure:''' | * '''Structure:''' | ||
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* '''Additional information:''' | * '''Additional information:''' | ||
| + | ** number of protein molecules per cell (minimal medium with glucose and ammonium): 164 {{PubMed|24696501}} | ||
=Biological materials = | =Biological materials = | ||
* '''Mutant:''' | * '''Mutant:''' | ||
| + | ** 1A942 ( ''mccB''::''kan''), {{PubMed|17056751}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A942&Search=1A942 BGSC] | ||
| + | ** 1A945 ( ''mccB''::''kan''), {{PubMed|17056751}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A945&Search=1A945 BGSC] | ||
* '''Expression vector:''' | * '''Expression vector:''' | ||
Latest revision as of 09:59, 17 April 2014
- Description: cystathionine lyase/ homocysteine gamma-lyase
| Gene name | mccB |
| Synonyms | yrhB |
| Essential | no |
| Product | cystathionine lyase/ homocysteine gamma-lyase |
| Function | methionine-to-cysteine conversion |
| Gene expression levels in SubtiExpress: mccB | |
| Metabolic function and regulation of this protein in SubtiPathways: mccB | |
| MW, pI | 40 kDa, 5.215 |
| Gene length, protein length | 1137 bp, 379 aa |
| Immediate neighbours | yrhC, mccA |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
biosynthesis/ acquisition of amino acids, membrane proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU27250
Phenotypes of a mutant
Database entries
- BsubCyc: BSU27250
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: L-cystathionine + H2O = L-cysteine + NH3 + 2-oxobutanoate (according to Swiss-Prot)
- Protein family: trans-sulfuration enzymes family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization:
- cell membrane (according to Swiss-Prot)
Database entries
- BsubCyc: BSU27250
- Structure:
- UniProt: O05394
- KEGG entry: [3]
- E.C. number: 4.4.1.1
Additional information
Expression and regulation
- Additional information:
- number of protein molecules per cell (minimal medium with glucose and ammonium): 164 PubMed
Biological materials
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
