brfA

brfA
168

release factor-dependent ribosome rescue factor

Locus
BSU_23540
Molecular weight
7.95 kDa
Isoelectric point
11.38
Protein length
Gene length
Function
resolution of stalled translation complexes
Product
ribosome rescue factor
Essential
no
E.C.
null
Synonyms
brfA, yqkK

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms

This gene is a member of the following regulons

Gene
Coordinates
2,451,148 → 2,451,363
Phenotypes of a mutant
a brfA SmpB double mutant has a severe growth defect PubMed
The protein
Catalyzed reaction/ biological activity
binds to non-stop stalled ribosomes, recruits homologous RF2, but not RF1, and induces its transition into an open active conformation PubMed
Structure
Biological materials
Mutant
MGNA-C457 (yqkK::erm), available at the NBRP B. subtilis, Japan
BKE23540 (ΔbrfA::erm  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CATGTGATCACTCCCTTTTT,  downstream forward: _UP4_TAGCATTCATCTCTATTGTT
BKK23540 (ΔbrfA::kan  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CATGTGATCACTCCCTTTTT,  downstream forward: _UP4_TAGCATTCATCTCTATTGTT
References
Reviews
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Research papers
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Time of last update: 2025-05-02 17:47:53

Author of last update: Jstuelk