serA

168

phosphoglycerate dehydrogenase

Locus: BSU_23070

Molecular weight: 56.95 kDa

Isoelectric point: 5.62

Protein length: 525 aa

Gene length: 1578 bp

Function: biosynthesis of serine

Product: phosphoglycerate dehydrogenase

Essential: no

E.C.: 1.1.1.95

Synonyms: serA

Genomic Context

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List of homologs in different organisms, belongs to COG1760 (Galperin et al., 2021)

Gene

Coordinates: 2,411,086 2,412,663

Phenotypes of a mutant

• auxotrophic for serine PubMed

The protein

Catalyzed reaction/ biological activity

• 3-phospho-D-glycerate + NAD⁺ --> 3-phosphooxypyruvate + H⁺ + NADH (according to UniProt)
• (R)-2-hydroxyglutarate + NAD⁺ --> 2-oxoglutarate + H⁺ + NADH (according to UniProt)

Protein family

• D-isomer specific 2-hydroxyacid dehydrogenase family (with YvcT and YoaD, according to UniProt)

Domains

• ACT domain (aa 452-524) (according to UniProt)

Structure

• 1YBA (PDB) (from E. coli, 30% identity, 52% similarity) PubMed
• P35136 (AlphaFold)

Modification

• S-cysteinylation after diamide stress (C410)PubMed
• active site Cys410 is S-bacillithiolated by NaOCl stress in B. subtilis and other Bacillus species PubMed PubMed

Localization

• membrane PubMed

Additional information

• belongs to the 100 most abundant proteins PubMed

Expression and Regulation

Operons

• Genes: serA

  Description: PubMed

  Regulation

  • strongly repressed in response to glucose starvation in M9 medium PubMed

  Regulatory mechanism

  • CcpA: activation, PubMed, in ccpA regulon

Biological materials

Mutant

• GP2392 ''serA::zeo'', available in Jrg Stlke's lab PubMed
• 1A614 (serA::erm), PubMed, available at BGSC and in Jrg Stlke's lab
• BKE23070 (serA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCGTTAGATTTCCTCCTA, downstream forward: _UP4_TAATTAAAAAAACTCAAGCT
• BKK23070 (serA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCGTTAGATTTCCTCCTA, downstream forward: _UP4_TAATTAAAAAAACTCAAGCT
• BP1273 (serA::kan trp+) available in Jörg Stülke's & Fabian Commichau's lab

LacZ fusion

• pGP187 (in pAC7), available in Jrg Stlke's lab
• pGP2287 (in pAC7), available in Jrg Stlke's lab

References

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