yodB

168

MarR/DUF24 family transcription repressor of azoR1, catD-catE and yodC expression

Locus: BSU_19540

Molecular weight: 12.71 kDa

Isoelectric point: 4.74

Protein length: 112 aa

Gene length: 339 bp

Function: regulation of quinone and diamide detoxification

Product: MarR/DUF24 family transcription repressor

Essential: no

Synonyms: yodB

Genomic Context

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List of homologs in different organisms, belongs to COG1733 (Galperin et al., 2021)

Gene

Coordinates: 2,127,345  2,127,683

Phenotypes of a mutant

• increased resistance to catechol, quinones and diamide PubMed

The protein

Protein family

• MarR/DUF24 family

Domains

• HTH hxlR-type domain (aa 6-105) (according to UniProt)

Structure

• 5HS7 (PDB) (reduced form) PubMed
• 5HS8 (PDB) (diamide-treated form) PubMed
• 5HS9 (PDB) (quinone-bound form) PubMed
• O34844 (AlphaFold)

Modification

• redox-controlled by intersubunit disulfide formation of Cys6 and Cys101' in response to quinones and diamide

Effectors of protein activity

• responds distinctly to diamide or methyl-p-benzoquinone PubMed

Paralogous protein(s)

• HypR, YkvN, HxlR, YdzF, CatR, YdeP, YtcD

Expression and Regulation

Operons

• Genes: yodB

  Description: PubMed

  Regulatory mechanism

  • YodB: repression, in yodB regulon

Biological materials

Mutant

• MGNA-B433 (yodB::erm), available at the NBRP B. subtilis, Japan
• BKE19540 (yodB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAAGTCTTCATCCCTTCA,  downstream forward: _UP4_TAAGGAAAGCGGCACCTGAA
• BKK19540 (yodB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAAGTCTTCATCCCTTCA,  downstream forward: _UP4_TAAGGAAAGCGGCACCTGAA

Labs working on this gene/protein

• Haike Antelmann,University of Greifswald, Germany

References

Reviews

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Original Publications

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