ywcA

168

similar to Na+-dependent acetate symporter

Locus: BSU_38240

Molecular weight: 54.79 kDa

Isoelectric point: 7.2

Protein length: 513 aa

Gene length: 1542 bp

Function: unknown

Product: unknown

Essential: no

E.C.: null

Synonyms: ywcA, ipa-31r

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG4147 (Galperin et al., 2021)

Gene

Coordinates: 3,924,225  3,925,766

Phenotypes of a mutant

• ''ywcB-ywcA'' mutant has slightly reduced acetic acid transport PubMed
• ''ywcB-ywcA'' mutant shows earlier pellicle biofilm formation PubMed

The protein

Catalyzed reaction/ biological activity

• the protein is involved in acetate transport PubMed
• the protein is not involved in proline transport  PubMed

Protein family

• sodium:solute symporter (SSF) (TC 2.A.21) family (according to UniProt)

Structure

• P39599 (AlphaFold)

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: ywcB-ywcA

  Regulation

  • expressed during sporulation (SigE) PubMed

  Sigma factors

  • SigE: sigma factor, PubMed, in sigE regulon

Biological materials

Mutant

• MGNA-B229 (ywcA::erm), available at the NBRP B. subtilis, Japan
• available in Erhard Bremer's lab PubMed
• BKE38240 (ywcA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AAATAATAAAAAAGCAGTCA,  downstream forward: _UP4_TAAAAAAAGCTCTCTTTATC
• BKK38240 (ywcA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AAATAATAAAAAAGCAGTCA,  downstream forward: _UP4_TAAAAAAAGCTCTCTTTATC

References

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