ptsA

168

PTS EIIA component

Locus: BSU_22230

Molecular weight: 17.79 kDa

Isoelectric point: 5.29

Protein length: 168 aa

Gene length: 507 bp

Function: uptake and phosphorylation of maltose, N-acetylglucosamine, sucrose, and trehalose

Product: PTS EIIA component

Essential: no

Synonyms: ptsA, ypqE

Genomic Context

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List of homologs in different organisms, belongs to COG2190 (Galperin et al., 2021)

Gene

Coordinates: 2,336,933  2,337,439

The protein

Protein family

• PTS permease, glucose family PubMed

Domains

• PTS EIIA domain type-1 (aa 36-140) (according to UniProt)

Structure

• 1AX3 (PDB) (IIA domain of PtsG, 46% identity) PubMed
• P50829 (AlphaFold)

Expression and Regulation

Operons

• Genes: ptsA

  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• MGNA-A477 (ypqE::erm), available at the NBRP B. subtilis, Japan
• QB6099 (aphA3), available in Jörg Stülke's lab PubMed
• BKE22230 (ypqE::ermC) (available at the BGSC and in Jörg Stülke's lab) PubMed
• GP1863 (ypqE::ermC) (available in Jörg Stülke's lab)
• BKE22230 (ptsA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAATGTATTTTCTCCCTTCT,  downstream forward: _UP4_TAAGCAGGGCGTATGCCTTG
• BKK22230 (ptsA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAATGTATTTTCTCCCTTCT,  downstream forward: _UP4_TAAGCAGGGCGTATGCCTTG

References

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