Difference between revisions of "MhqE"

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(Basic information/ Evolution)
 
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|style="background:#ABCDEF;" align="center"|'''Function''' || resistance to methyl hydroquinone and catechol
 
|style="background:#ABCDEF;" align="center"|'''Function''' || resistance to methyl hydroquinone and catechol
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU19570 mhqE]
 
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 33 kDa, 5.041   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 33 kDa, 5.041   
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|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 909 bp, 303 aa  
 
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 909 bp, 303 aa  
 
|-
 
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[mhqD]]'', ''[[yodF]]''
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[mhqD]]'', ''[[yoyD]]''
 
|-
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB13848&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU19570 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU19570 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU19570 DNA_with_flanks]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:yodE_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:yodE_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yodE_2129082_2129993_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:mhqE_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU19570]]
 
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__TOC__
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=== Database entries ===
 
=== Database entries ===
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* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU19570&redirect=T BSU19570]
  
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/mhqED.html]
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/mhqED.html]
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* '''Catalyzed reaction/ biological activity:'''  
 
* '''Catalyzed reaction/ biological activity:'''  
  
* '''Protein family:'''
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* '''Protein family:''' extradiol ring-cleavage dioxygenase family (according to UniProt)
  
 
* '''Paralogous protein(s):''' [[MhqA]], [[MhqO]]
 
* '''Paralogous protein(s):''' [[MhqA]], [[MhqO]]
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=== Database entries ===
 
=== Database entries ===
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* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU19570&redirect=T BSU19570]
  
 
* '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?pdbId=3OAJ 3OAJ] ([[MhqO]], 48% identical residues)
 
* '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?pdbId=3OAJ 3OAJ] ([[MhqO]], 48% identical residues)

Latest revision as of 08:58, 25 June 2019

  • Description: dioxygenase/glyoxalase

Gene name mhqE
Synonyms yodE
Essential no
Product dioxygenase/glyoxalase
Function resistance to methyl hydroquinone and catechol
Gene expression levels in SubtiExpress: mhqE
MW, pI 33 kDa, 5.041
Gene length, protein length 909 bp, 303 aa
Immediate neighbours mhqD, yoyD
Sequences Protein DNA DNA_with_flanks
Genetic context
YodE context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
MhqE expression.png
























Categories containing this gene/protein

resistance against oxidative and electrophile stress

This gene is a member of the following regulons

MhqR regulon

The gene

Basic information

  • Locus tag: BSU19570

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: extradiol ring-cleavage dioxygenase family (according to UniProt)

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 3OAJ (MhqO, 48% identical residues)
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Stefanie Töwe, Montira Leelakriangsak, Kazuo Kobayashi, Nguyen Van Duy, Michael Hecker, Peter Zuber, Haike Antelmann
The MarR-type repressor MhqR (YkvE) regulates multiple dioxygenases/glyoxalases and an azoreductase which confer resistance to 2-methylhydroquinone and catechol in Bacillus subtilis.
Mol Microbiol: 2007, 66(1);40-54
[PubMed:17725564] [WorldCat.org] [DOI] (P p)

Van Duy Nguyen, Carmen Wolf, Ulrike Mäder, Michael Lalk, Peter Langer, Ulrike Lindequist, Michael Hecker, Haike Antelmann
Transcriptome and proteome analyses in response to 2-methylhydroquinone and 6-brom-2-vinyl-chroman-4-on reveal different degradation systems involved in the catabolism of aromatic compounds in Bacillus subtilis.
Proteomics: 2007, 7(9);1391-408
[PubMed:17407181] [WorldCat.org] [DOI] (P p)