ylnF

168

probably precorrin-2 dehydrogenase

Locus: BSU_15630

Molecular weight: 17.86 kDa

Isoelectric point: 9.24

Protein length: 162 aa

Gene length: 489 bp

Function: siroheme biosynthesis , sulfite reduction

Product: probably precorrin-2 dehydrogenase

Essential: no

Synonyms: ylnF, sirC

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG1648 (Galperin et al., 2021)

Gene

Coordinates: 1,635,603  1,636,091

The protein

Catalyzed reaction/ biological activity

• NAD+ + precorrin-2 --> 2 H⁺ + NADH + sirohydrochlorin (according to UniProt)

Protein family

• ferrochelatase family (with HemH, according to UniProt)

Structure

• 3DFZ (PDB) (36% identity) PubMed
• O34813 (AlphaFold)

Expression and Regulation

Operons

• Genes: cysH-cysP-sat-cysC-ylnD-sirB-ylnF

  Description: PubMed

  Regulation

  • induced by methionine starvation (S-box) PubMed
  • the S-box RNA is degraded by RNase Y PubMed

  Regulatory mechanism

  • CymR: transcription repression, [pubmed|], in cymR regulon
  • S-box: RNA switch, , the S-box riboswitch binds S-adenosylmethionine resulting in termination PubMed, in S-box

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• MGNA-B370 (ylnF::erm), available at the NBRP B. subtilis, Japan
• BKE15630 (ylnF::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AATCACAACCTTCTTCTTTT,  downstream forward: _UP4_TAAGCCAGAGCATACAAATG
• BKK15630 (ylnF::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AATCACAACCTTCTTCTTTT,  downstream forward: _UP4_TAAGCCAGAGCATACAAATG

References

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