panE

168

ketopantoate reductase

Locus: BSU_15110

Molecular weight: 33.13 kDa

Isoelectric point: 5.86

Protein length: 298 aa

Gene length: 897 bp

Function: biosynthesis of coenzyme A

Product: ketopantoate reductase

Essential: no

E.C.: 1.1.1.169

Synonyms: ylbQ

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG1893 (Galperin et al., 2021)

Gene

Coordinates: 1,577,409  1,578,305

Phenotypes of a mutant

• the mutant tends to acquire suppressor mutations that result in improved growth PubMed

The protein

Catalyzed reaction/ biological activity

• ketopantoate + H⁺ + NADPH --> (R)-pantoate + NADP⁺ PubMed

Protein family

• ketopantoate reductase family (with PanG, according to UniProt)

Structure

• 3EGO (PDB)
• O34661 (AlphaFold)

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: panE-bshC

  Description: PubMed

  Regulation

  • ''ylbQ'': induced by diamide stess (thiol depletion) (Spx) PubMed

  Regulatory mechanism

  • Spx: activation, PubMed, in spx regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• MGNA-B254 (ylbQ::erm), available at the NBRP B. subtilis, Japan
• BKE15110 (panE::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGATGTCACACCCAATTT,  downstream forward: _UP4_TGAGCTTTTTCGGTAACATG
• BKK15110 (panE::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGATGTCACACCCAATTT,  downstream forward: _UP4_TGAGCTTTTTCGGTAACATG
• GP3384 (ΔpanE::neo trpC2), available in Jörg Stülke's lab PubMed

References

Original Publications

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