yflK

168

similar to hydroxylaminopurine resistance protein

locus: BSU_07650

Molecular weight: 24.69 kDa

pI: 7.76

Protein length: 221 aa

Gene length: 666 bp

function: unknown

product: unknown

essential: no

synonyms: yflK

List of homologs in different organisms, belongs to COG2258 (Galperin et al., 2021)

Gene

Coordinates: 838,077 → 838,742

The protein

[wiki|Domains]

• MOSC domain (aa 33-167) (according to UniProt)

Structure

• [PDB|5YHH] (from Geobacillus stearothermophilus, 50% identity) [pubmed|29459651]
• [AF|O34542]

Expression and Regulation

Operons

• genes: [gene|B51ADFB53CA54309D866BB089502639E9571238B|nos]-[gene|C2E28821689A07B9AA1B0BD5C84D856C04D83E95|yflK]

  description: [Pubmed|22383849]

  additional information

  • Note that the [gene|D7C46835B9984E2C5E375816F2BB16566202CF27|yflL] is located between [gene|B51ADFB53CA54309D866BB089502639E9571238B|nos] and [gene|C2E28821689A07B9AA1B0BD5C84D856C04D83E95|yflK], but on the opposite strand

Biological materials

Mutant

• MGNA-C335 (yflK::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2333 NBRP B. subtilis, Japan]
• BKE07650 (Δ[gene|C2E28821689A07B9AA1B0BD5C84D856C04D83E95|yflK]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE07650 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATACAAACACATCCCCTTT,  downstream forward: _UP4_TAGAAAAAAGCCGCGCATAT
• BKK07650 (Δ[gene|C2E28821689A07B9AA1B0BD5C84D856C04D83E95|yflK]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK07650 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATACAAACACATCCCCTTT,  downstream forward: _UP4_TAGAAAAAAGCCGCGCATAT

References

• 11886751,29459651