panB

168

alpha-ketoisovalerate hydroxymethyltransferase

Locus: BSU_22430

Molecular weight: 29.61 kDa

Isoelectric point: 5.28

Protein length: 277 aa

Gene length: 834 bp

Function: biosynthesis of coenzyme A

Product: alpha-ketoisovalerate hydroxymethyltransferase

Essential: quasi

E.C.: 2.1.2.11

Synonyms: panB

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0413 (Galperin et al., 2021)

Gene

Coordinates: 2,353,839  2,354,672

Phenotypes of a mutant

• quasi-essential, the mutant is auxotrophic for pantothenate PubMed
• the mutant is viable if it acquires mutations (cymR or cysK) that allow constitutive expression of tcyJ-tcyK-tcyL-tcyM-tcyN for the uptake of cysteinopantetheine PubMed

The protein

Catalyzed reaction/ biological activity

• (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + alpha-ketoisovalerate + H₂O --> (6S)-5,6,7,8-tetrahydrofolate + ketopantoate PubMed

Protein family

• PanB family (single member, according to UniProt)

Structure

• 1M3U (PDB) (from E. coli, 48% identity, complex with the product ketopantoate) PubMed
• P52996 (AlphaFold)

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: panB-panC-panD-dinG-ypmA-tseB-aspB

  Description: PubMed

Biological materials

Mutant

• GP4401 (ΔpanB::neo), available in Jörg Stülke's lab PubMed
• PA126 (panB::cat), available in Jörg Stülke's lab
• BKE22430 (panB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTTTTCTCCTCCTCATG,  downstream forward: _UP4_GTGCTTGACGGCTTGTACGG
• BKK22430 (panB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTTTTCTCCTCCTCATG,  downstream forward: _UP4_GTGCTTGACGGCTTGTACGG

References

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