pyrP

168

uracil permease

Locus: BSU_15480

Molecular weight: 45.41 kDa

Isoelectric point: 9.5

Protein length: 435 aa

Gene length: 1308 bp

Function: uracil transport in/out via proton symport

Product: uracil permease

Essential: no

Synonyms: pyrP

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG2233 (Galperin et al., 2021)

Gene

Coordinates: 1,619,023  1,620,330

The protein

Protein family

• xanthine/uracil permease family (according to UniProt)
• Nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) subfamily (according to UniProt)

Structure

• 3QE7 (PDB) (E. coli uracil transporter, 43% identity) PubMed
• P39766 (AlphaFold)

Localization

• cell membrane PubMed

Expression and Regulation

Operons

• Genes: pyrR-pyrP-pyrB-pyrC-pyrAA-pyrAB-pyrK-pyrD-pyrF-pyrE

  Description: PubMed

  Regulation

  • induced in the absence of uridine nucleotides (PyrR) PubMed

  Regulatory mechanism

  • PyrR: termination, via RNA switch, in pyrR regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• BKE15480 (pyrP::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGATGATTTCCCCCTGAT,  downstream forward: _UP4_ACATCTGAACAACATCATAT
• BKK15480 (pyrP::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGATGATTTCCCCCTGAT,  downstream forward: _UP4_ACATCTGAACAACATCATAT
• BP1258 (pyrP::cat trp+) available in Jörg Stülke's & Fabian Commichau's lab

References

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