aexA

168

"sleeping beauty" amino acid exporter

Locus: BSU_05160

Molecular weight: 35.16 kDa

Isoelectric point: 9.62

Protein length: 319 aa

Gene length: 960 bp

Function: export of toxic/ growth-inhibiting amino acids

Product: amino acid exporter A

Essential: no

E.C.: null

Synonyms: ydeD

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0697 (Galperin et al., 2021)

Gene

Coordinates: 562,502  563,461

The protein

Catalyzed reaction/ biological activity

• export of asparagine PubMed
• export of L-alanine PubMed
• export of ß-alanine PubMed
• export of serine PubMed
• export of glycine PubMed

Protein family

• EamA transporter family (according to UniProt)

Domains

• 2EamA domains (aa 18-149, aa 171-296) (according to UniProt)

Structure

• P96661 (AlphaFold)

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: aexA

  Regulation

  • (AerA): transcription activation PubMed

  Expression

  • weak induction in the presence of 2,3-diaminopropionic acid (AerA) PubMed
  • very strong constitutive expression if AerA acquires mutations under selective pressure by toxic amino acids PubMed

  Regulatory mechanism

  • AerA: transcription activation, PubMed, in aerA regulon

Biological materials

Mutant

• GP3955 (aexA::cat), available in Jörg Stülke's lab PubMed
• MGNA-C080 (ydeD::erm), available at the NBRP B. subtilis, Japan
• BKE05160 (aexA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATACATGACTCCTCACTAA,  downstream forward: _UP4_TAGGGGCTTTTTTTGCTTAC
• BKK05160 (aexA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATACATGACTCCTCACTAA,  downstream forward: _UP4_TAGGGGCTTTTTTTGCTTAC

Expression system

• GP4812 (trpC2 xkdE::P_xyl-aexA-ermC), available in Jörg Stülke's lab

Expression vectors

• pGP3727: expression of aexA (gapA RBS) by pBQ200 in B. subtilis, available in Jörg Stülke's lab PubMed

LacZ fusion

• GP4132, cat, (based on pAC6), available in Jörg Stülke's lab PubMed

References

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