glnK

168

two-component sensor kinase, regulation of the glsA-glnT operon

Locus: BSU_02440

Molecular weight: 44.00 kDa

Isoelectric point: 5.73

Protein length: 410 aa

Gene length: 1233 bp

Function: regulation of the glsA-glnT operon

Product: two-component sensor kinase

Essential: no

Synonyms: glnK, ycbA, yzgA

Genomic Context

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List of homologs in different organisms, belongs to COG0642 (Galperin et al., 2021)

Gene

Coordinates: 265,476 → 266,708

The protein

Catalyzed reaction/ biological activity

• autophosphorylation, phosphorylation of GlnL
• ATP + protein L-histidine --> ADP + protein N-phospho-L-histidine (according to UniProt)

Domains

• four transmembrane segments
• Histidine kinase domain (aa 189-405) (according to UniProt)

Structure

• P40758 (AlphaFold)

Modification

• autophosphorylation on a His residue

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: glnK-glnL

  Description: PubMed

Biological materials

Mutant

• BKE02440 (ΔglnK::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGAGGGCCAGTATATATC,  downstream forward: _UP4_ATTCAGAAAGGGTGAACCAA
• BKK02440 (ΔglnK::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGAGGGCCAGTATATATC,  downstream forward: _UP4_ATTCAGAAAGGGTGAACCAA

References

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