hmoB

168

heme monooxygenase

Locus: BSU_10100

Molecular weight: 18.68 kDa

Isoelectric point: 5.22

Protein length: 166 aa

Gene length: 501 bp

Function: degradation of heme, acquisition of iron

Product: heme monooxygenase

Essential: no

E.C.: null

Synonyms: hmoB, yixC, yhgC

Genomic Context

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List of homologs in different organisms, belongs to COG2329 (Galperin et al., 2021)

Gene

Coordinates: 1,083,229  1,083,729

The protein

Catalyzed reaction/ biological activity

• binds hemin in vitro with ~1:1 stoichiometry and degrade hemin in the presence of an electron donor PubMed
• heme b + 3 O₂ + 3 reduced [NADPH—hemoprotein reductase] --> biliverdin IXα + CO + Fe²⁺ + H⁺ + 3 H₂O + 3 oxidized [NADPH—hemoprotein reductase] (according to UniProt)

Protein family

• antibiotic biosynthesis monooxygenase family (with HmoA, according to UniProt)

Domains

• ABM domain (aa 66-153) (according to UniProt)

Structure

• 3TVZ (PDB) PubMed
• P38049 (AlphaFold)

Expression and Regulation

Operons

• Genes: hmoB

  Description: PubMed

  Regulation

  • constitutively expressed PubMed

Biological materials

Mutant

• MGNA-B503 (yhgC::erm), available at the NBRP B. subtilis, Japan
• BKE10100 (hmoB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATCAGCCACCTCCTGCT,  downstream forward: _UP4_TAGCTGCCGGCATGTCCGGC
• BKK10100 (hmoB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATCAGCCACCTCCTGCT,  downstream forward: _UP4_TAGCTGCCGGCATGTCCGGC

References

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