lonB

168

Lon-like ATP-dependent protease

Locus: BSU_28210

Molecular weight: 60.26 kDa

Isoelectric point: 6.27

Protein length: 552 aa

Gene length: 1659 bp

Function: protein quality control

Product: Lon-like ATP-dependent protease

Essential: no

Synonyms: lonB, ysxF

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG1067 (Galperin et al., 2021)

Gene

Coordinates: 2,882,971 → 2,884,629

The protein

Catalyzed reaction/ biological activity

• Hydrolysis of proteins in presence of ATP (according to UniProt)

Protein family

• Peptidase S16 family (with DdcP and LonA, according to UniProt)

Domains

• Lon proteolytic (aa 349-535) (according to UniProt)

Structure

• 6U5Z (PDB) (from E. coli, corresponds to aa 93 ... 538, 27% identity)
• P42425 (AlphaFold)

Localization

• localized to the forespore membrane early in development, followed by localization throughout the forespore later in development PubMed

Expression and Regulation

Operons

• Genes: lonB

  Description: PubMed

  Regulation

  • expressed early during sporulation in the forespore (SigF) PubMed

  Sigma factors

  • SigF: sigma factor, PubMed, in sigF regulon

Biological materials

Mutant

• BKE28210 (ΔlonB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGGTGGTCCCTCCTGAAA,  downstream forward: _UP4_TAAACCCTATTTTGATAATA
• BKK28210 (ΔlonB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGGTGGTCCCTCCTGAAA,  downstream forward: _UP4_TAAACCCTATTTTGATAATA

References

Reviews

Loading

Original Publications

Loading