rph

168

RNase PH, 3-5 exoribonuclease

Locus: BSU_28370

Molecular weight: 26.53 kDa

Isoelectric point: 4.89

Protein length: 245 aa

Gene length: 738 bp

Function: 3-5 exoribonuclease

Product: RNase PH (EC 2.7.7.56)

Essential: no

E.C.: 2.7.7.56

Synonyms: rph

Genomic Context

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Categories containing this gene/protein

Information processing, RNA synthesis and degradation, RNases, Exoribonucleases

Information processing, Protein synthesis, modification and degradation, Translation, rRNA modification and maturation

Information processing, Protein synthesis, modification and degradation, Translation, tRNA modification and maturation

List of homologs in different organisms, belongs to COG0689 (Galperin et al., 2021)

Gene

Coordinates: 2,901,154  2,901,891

The protein

Catalyzed reaction/ biological activity

major player in the exonucleolytic maturation of CCA-containing tRNA precursors PubMed
responsible for endonucleolytic cleavage of scRNA (''scr'') PubMed
phosphate + tRNA_(n+1) --> ribonucleoside 5'-diphosphate + tRNA_(n) (according to UniProt)

Protein family

RNase PH family (single member, according to UniProt)

Structure

1OYS (PDB)
P28619 (AlphaFold)

Expression and Regulation

Operons

Genes: rph-ysnA-ysnB

Description: PubMed

Biological materials

Mutant

BKE28370 (rph::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTTTTACCTCCGATTGT,  downstream forward: _UP4_TAAAGACAGAAAGGCTTGAT
BKK28370 (rph::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTTTTACCTCCGATTGT,  downstream forward: _UP4_TAAAGACAGAAAGGCTTGAT

Labs working on this gene/protein

David Bechhofer, Mount Sinai School, New York, USA Homepage
Ciaran Condon, IBPC, Paris, France Homepage

References

Reviews

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Original Publications

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