phy

168

phytase

Locus: BSU_19800

Molecular weight: 41.79 kDa

Isoelectric point: 4.94

Protein length: 382 aa

Gene length: 1149 bp

Function: utilization of inositol hexakisphosphate (phytate)

Product: 3-phytase

Essential: no

E.C.: 3.1.3.8

Synonyms: phy, yodV, yzxA

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG4247 (Galperin et al., 2021)

Gene

Coordinates: 2,150,108 → 2,151,256

The protein

Catalyzed reaction/ biological activity

• 1D-myo-inositol hexakisphosphate + H₂O --> 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate (according to UniProt)

Domains

• BPP domain (aa 27-361) (according to UniProt)

Structure

• 3AMR (PDB)
• P42094 (AlphaFold)

Localization

• extracellular (signal peptide) PubMed

Expression and Regulation

Operons

• Genes: phy

  Description: PubMed

  Regulatory mechanism

  • AbrB: repression, PubMed, in abrB regulon

  Additional information

  • the gene is not expressed in 'B. subtilis' due to the absene of a functional promoter PubMed

Biological materials

Mutant

• MGNA-B509 (yodV::erm), available at the NBRP B. subtilis, Japan
• BKE19800 (Δphy::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTGGTACCCTCCTCTTT,  downstream forward: _UP4_TAGAATAGAAAGCAGCTTGT
• BKK19800 (Δphy::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTGGTACCCTCCTCTTT,  downstream forward: _UP4_TAGAATAGAAAGCAGCTTGT

References

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