lipB

168

extracellular esterase, lipase

Locus: BSU_08350

Molecular weight: 22.22 kDa

Isoelectric point: 9.68

Protein length: 210 aa

Gene length: 633 bp

Function: lipid degradation

Product: extracellular esterase, lipase

Essential: no

Synonyms: lipB, yfiP

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG1075 (Galperin et al., 2021)

Gene

Coordinates: 910,019  910,651

The protein

Catalyzed reaction/ biological activity

• triacylglycerol + H₂O --> diacylglycerol + fatty acid + H⁺ (according to UniProt)

Protein family

• AB hydrolase superfamily (according to UniProt)

Structure

• 2QXT (PDB) (Lip, 75% identity) PubMed
• Q79F14 (AlphaFold)

Paralogous protein(s)

• Lip

Expression and Regulation

Operons

• Genes: lipB

  Description: PubMed

Biological materials

Mutant

• MGNA-C304 (lipB::erm), available at the NBRP B. subtilis, Japan
• BKE08350 (lipB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACGTTTATTCCCCCAAAAA,  downstream forward: _UP4_TAATATCTTCAAAAAACAAC
• BKK08350 (lipB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACGTTTATTCCCCCAAAAA,  downstream forward: _UP4_TAATATCTTCAAAAAACAAC

References

Loading