queC

168

synthesis of the modified ribonucleotide queuosine

Locus: BSU_13720

Molecular weight: 24.38 kDa

Isoelectric point: 5.15

Protein length: 219 aa

Gene length: 660 bp

Function: tRNA modification

Product: unknown

Essential: no

Synonyms: queC, ykvJ

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0603 (Galperin et al., 2021)

Gene

Coordinates: 1,439,448  1,440,107

The protein

Catalyzed reaction/ biological activity

• 7-carboxy-7-deazaguanine + ATP + NH₄⁺ --> 7-cyano-7-deazaguanine + ADP + H⁺ + H₂O + phosphate (according to UniProt)

Protein family

• queC family (single member, according to UniProt)

Structure

• 3BL5 (PDB) PubMed
• O31675 (AlphaFold)

Expression and Regulation

Operons

• Genes: queC-queD-queE-queF

  Description: PubMed

  Regulation

  • repressed in the presence of queuosine (preQ1 riboswitch) PubMed

  Regulatory mechanism

  • preQ1 riboswitch: RNA switch, in the absence of queuosine PubMed, in preQ1 riboswitch

Biological materials

Mutant

• MGNA-A789 (ykvJ::erm), available at the NBRP B. subtilis, Japan
• BKE13720 (queC::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGATTCCTCTCCTTCTCC,  downstream forward: _UP4_GAATATATGGTGATGAAAGG
• BKK13720 (queC::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGATTCCTCTCCTTCTCC,  downstream forward: _UP4_GAATATATGGTGATGAAAGG

References

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