Difference between revisions of "MhqA"
(10 intermediate revisions by 3 users not shown) | |||
Line 1: | Line 1: | ||
* '''Description:''' hydroquinone-specific dioxygenase, confers resistence to methyl-hydroxyquinone <br/><br/> | * '''Description:''' hydroquinone-specific dioxygenase, confers resistence to methyl-hydroxyquinone <br/><br/> | ||
− | |||
{| align="right" border="1" cellpadding="2" | {| align="right" border="1" cellpadding="2" | ||
|- | |- | ||
Line 13: | Line 12: | ||
|- | |- | ||
|style="background:#ABCDEF;" align="center"|'''Function''' || resistence to methyl-hydroxyquinone | |style="background:#ABCDEF;" align="center"|'''Function''' || resistence to methyl-hydroxyquinone | ||
+ | |- | ||
+ | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU12870 mhqA] | ||
|- | |- | ||
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 35 kDa, 5.404 | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 35 kDa, 5.404 | ||
Line 20: | Line 21: | ||
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[steT]]'', ''[[ykcB]]'' | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[steT]]'', ''[[ykcB]]'' | ||
|- | |- | ||
− | | | + | |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU12870 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU12870 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU12870 DNA_with_flanks] |
|- | |- | ||
|- | |- | ||
Line 26: | Line 27: | ||
|colspan="2" | '''Genetic context''' <br/> [[Image:ykcA_context.gif]] | |colspan="2" | '''Genetic context''' <br/> [[Image:ykcA_context.gif]] | ||
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div> | <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div> | ||
+ | |- | ||
+ | |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=mhqA_1353080_1354030_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:mhqA_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU12870]] | ||
|- | |- | ||
|} | |} | ||
__TOC__ | __TOC__ | ||
+ | <br/><br/><br/><br/> | ||
+ | <br/><br/><br/><br/> | ||
+ | <br/><br/><br/><br/> | ||
+ | <br/><br/><br/><br/> | ||
+ | <br/><br/><br/><br/> | ||
+ | |||
<br/><br/> | <br/><br/> | ||
Line 44: | Line 53: | ||
* '''Locus tag:''' BSU12870 | * '''Locus tag:''' BSU12870 | ||
− | |||
− | |||
===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
=== Database entries === | === Database entries === | ||
+ | * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU12870&redirect=T BSU12870] | ||
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/mhqA.html] | * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/mhqA.html] | ||
Line 66: | Line 74: | ||
* '''Catalyzed reaction/ biological activity:''' | * '''Catalyzed reaction/ biological activity:''' | ||
− | * '''Protein family:''' extradiol ring-cleavage dioxygenase family (according to | + | * '''Protein family:''' extradiol ring-cleavage dioxygenase family (according to UniProt) |
* '''Paralogous protein(s):''' [[MhqE]], [[MhqO]] | * '''Paralogous protein(s):''' [[MhqE]], [[MhqO]] | ||
Line 87: | Line 95: | ||
=== Database entries === | === Database entries === | ||
+ | * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU12870&redirect=T BSU12870] | ||
* '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?pdbId=3OAJ 3OAJ] ([[MhqO]], 34% identical residues) | * '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?pdbId=3OAJ 3OAJ] ([[MhqO]], 34% identical residues) | ||
Line 102: | Line 111: | ||
* '''Operon:''' ''mhqA'' {{PubMed|17725564}} | * '''Operon:''' ''mhqA'' {{PubMed|17725564}} | ||
− | * '''[ | + | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=mhqA_1353080_1354030_1 mhqA] {{PubMed|22383849}} |
+ | |||
+ | * '''Sigma factor:''' [[SigA]] {{PubMed|17725564}} | ||
* '''Regulation:''' | * '''Regulation:''' | ||
Line 111: | Line 122: | ||
* '''Additional information:''' | * '''Additional information:''' | ||
+ | ** number of protein molecules per cell (minimal medium with glucose and ammonium): 183 {{PubMed|24696501}} | ||
=Biological materials = | =Biological materials = |
Latest revision as of 08:58, 25 June 2019
- Description: hydroquinone-specific dioxygenase, confers resistence to methyl-hydroxyquinone
Gene name | mhqA |
Synonyms | ykcA |
Essential | no |
Product | hydroquinone-specific dioxygenase |
Function | resistence to methyl-hydroxyquinone |
Gene expression levels in SubtiExpress: mhqA | |
MW, pI | 35 kDa, 5.404 |
Gene length, protein length | 948 bp, 316 aa |
Immediate neighbours | steT, ykcB |
Sequences | Protein DNA DNA_with_flanks |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
resistance against oxidative and electrophile stress
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU12870
Phenotypes of a mutant
Database entries
- BsubCyc: BSU12870
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: extradiol ring-cleavage dioxygenase family (according to UniProt)
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: cytoplasm (according to Swiss-Prot)
Database entries
- BsubCyc: BSU12870
- UniProt: O34689
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Operon: mhqA PubMed
- Additional information:
- number of protein molecules per cell (minimal medium with glucose and ammonium): 183 PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Stefanie Töwe, Montira Leelakriangsak, Kazuo Kobayashi, Nguyen Van Duy, Michael Hecker, Peter Zuber, Haike Antelmann
The MarR-type repressor MhqR (YkvE) regulates multiple dioxygenases/glyoxalases and an azoreductase which confer resistance to 2-methylhydroquinone and catechol in Bacillus subtilis.
Mol Microbiol: 2007, 66(1);40-54
[PubMed:17725564]
[WorldCat.org]
[DOI]
(P p)
Van Duy Nguyen, Carmen Wolf, Ulrike Mäder, Michael Lalk, Peter Langer, Ulrike Lindequist, Michael Hecker, Haike Antelmann
Transcriptome and proteome analyses in response to 2-methylhydroquinone and 6-brom-2-vinyl-chroman-4-on reveal different degradation systems involved in the catabolism of aromatic compounds in Bacillus subtilis.
Proteomics: 2007, 7(9);1391-408
[PubMed:17407181]
[WorldCat.org]
[DOI]
(P p)