yitI

168

similar to glyphosate N-acetyltransferase

locus: BSU_11000

Molecular weight: 17.47 kDa

pI: 9.17

Protein length: 154 aa

Gene length: 465 bp

function: unknown

product: unknown

essential: no

ec: 2.3.1.-

synonyms: yitI

List of homologs in different organisms, belongs to COG2153 (Galperin et al., 2021)

Gene

Coordinates: 1,178,218  1,178,682

The protein

Protein family

• [wiki|Acetyltransferase family] (according to UniProt)

[wiki|Domains]

• [wiki|N-acetyltransferase domain] (aa 2-146) (according to UniProt)

Structure

• [PDB|2JDC] (from B. licheniformis, 59% identity) [pubmed|17272278]
• [AF|O06744]

Expression and Regulation

Operons

• genes: [gene|2751630B6472346B19FB20F57F16013D0FACA9F7|yitI]-[gene|1A31885756344F8BD2BED4E4526D43D6D5AB85F3|yitH]

  description: [pubmed|22383849]

Biological materials

Mutant

• MGNA-B205 (yitI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1204 NBRP B. subtilis, Japan]
• BKE11000 ([gene|2751630B6472346B19FB20F57F16013D0FACA9F7|yitI]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE11000 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGATTGTTTTTCTATATC,  downstream forward: _UP4_TGATCTTATGGACGTAGTAG
• BKK11000 ([gene|2751630B6472346B19FB20F57F16013D0FACA9F7|yitI]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK11000 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGATTGTTTTTCTATATC,  downstream forward: _UP4_TGATCTTATGGACGTAGTAG

References

• 19258532,17272278, 15155947