nsrR

168

nitric oxide-responsive regulator

Locus: BSU_09380

Molecular weight: 16.47 kDa

Isoelectric point: 7.75

Protein length: 146 aa

Gene length: 441 bp

Function: repression of ResD-ResE-dependent genes in the absence of nitric oxide (NO)

Product: transcriptional repressor

Essential: no

Synonyms: nsrR, yhdE

Genomic Context

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Categories containing this gene/protein

Information processing, Regulation of gene expression, Transcription factors and their control, Transcription factors/ other

Lifestyles, Coping with stress, Resistance against other toxic compounds (nitric oxide, phenolic acids, flavonoids, oxalate)

List of homologs in different organisms, belongs to COG1959 (Galperin et al., 2021)

This gene is a member of the following regulons

NsrR regulon

Gene

Coordinates: 1,013,411 → 1,013,851

The protein

Domains

HTH rrf2-type domain (aa 2-133) (according to UniProt)

Cofactors

Fe-S cluster, required for DNA-binding PubMed

Structure

3LWF (PDB) (from Listeria innocua, 29% identity)
O07573 (AlphaFold)

Effectors of protein activity

nitric oxide (NO) acts as molecular inducer and results in NsrR release from its DNA targets PubMed
[4Fe-4S]-NsrR binds around the -35 element of the ''nasD'' promoter with much higher affinity than apo-NsrR and binding of [4Fe-4S]-NsrR, but not apo-protein, is sensitive to NO PubMed

Expression and Regulation

Operons

Genes: nsrR

Description: PubMed

Biological materials

Mutant

MGNA-A692 (yhdE::erm), available at the NBRP B. subtilis, Japan
BKE09380 (ΔnsrR::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAATAAGACCTCAGAATA,  downstream forward: _UP4_TAGATTAAGATTCCTTCTTT
BKK09380 (ΔnsrR::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAATAAGACCTCAGAATA,  downstream forward: _UP4_TAGATTAAGATTCCTTCTTT

References

Reviews

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Other original publications

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The NsrR regulon

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