yetO

yetO
168

cytochrome P450 (CYP102A2)/ NADPH-cytochrome P450 reductase

Locus
BSU_07250
Molecular weight
119.26 kDa
Isoelectric point
5.73
Protein length
Gene length
Function
fatty acid metabolism
Product
NADPH-cytochrome P450 reductase
Essential
no
E.C.
1.6.2.4
Synonyms
yetO, yfnJ

Genomic Context

List of homologs in different organisms, belongs to COG2124 (Galperin et al., 2021)

Gene
Coordinates
792,682  795,867
The protein
Catalyzed reaction/ biological activity
oxidation of even- and odd-chain saturated and unsaturated fatty acids to the respective omega-3, omega-2 and omega-1 hydroxylated fatty acids PubMed
alkane + O2 + reduced [NADPH—hemoprotein reductase] --> primary alcohol + H+ + H2O + oxidized [NADPH—hemoprotein reductase] (according to UniProt)
NADPH + 2 oxidized [cytochrome P450] --> H+ + NADP+ + 2 reduced [cytochrome P450] (according to UniProt)
Protein family
cytochrome P450 family (according to UniProt)
Flavodoxin-like domain (aa 493-632) (according to UniProt)
FAD-binding FR-type domain (aa 671-904) (according to UniProt)
FAD (according to UniProt)
Structure
2X7Y (PDB) (from Bacillus megaterium; 66% identity, 87% similarity)  PubMed
Paralogous protein(s)
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
Genes
Description
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yetO

2025-07-22 23:01:56

Jstuelk

75

3464780681882c900eb43da61a692e9ac55909d5

6A780364E857EE722D7FBA2C73BB7BB800B08977

Biological materials
Mutant
MGNA-C225 (yfnJ::erm), available at the NBRP B. subtilis, Japan
BKE07250 (yetO::erm  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CATCGAACCATTCCTTTCCG,  downstream forward: _UP4_TAGATAAAGAAGACTGGAGA
BKK07250 (yetO::kan  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CATCGAACCATTCCTTTCCG,  downstream forward: _UP4_TAGATAAAGAAGACTGGAGA
References
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Page visits: 4766

Time of last update: 2025-07-28 17:27:51

Author of last update: Melvin.boenninger