mhqN

168

similar to NAD(P)H nitroreductase

Locus: BSU_05480

Molecular weight: 23.41 kDa

Isoelectric point: 5.56

Protein length: 206 aa

Gene length: 621 bp

Function: unknown

Product: unknown

Essential: no

Synonyms: mhqN, ydfN

Genomic Context

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Categories containing this gene/protein

Lifestyles, Coping with stress, Resistance against oxidative and electrophile stress

List of homologs in different organisms, belongs to COG0778 (Galperin et al., 2021)

This gene is a member of the following regulons

SigA regulon, MhqR regulon

Gene

Coordinates: 596,478  597,098

The protein

Protein family

nitroreductase family (according to UniProt)

Structure

3BEM (PDB)
P96692 (AlphaFold)

Expression and Regulation

Operons

Genes: mhqN-mhqO-mhqP

Description: PubMed

Regulation

induced by catechol and 2-methylhydroquinone (MhqR) PubMed

Regulatory mechanism

MhqR: repression, PubMed, in mhqR regulon

Sigma factors

SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

MGNA-C153 (ydfN::erm), available at the NBRP B. subtilis, Japan
BKE05480 (mhqN::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTACGCTACACTCCCTTT,  downstream forward: _UP4_TATATGTAAAAGGAGCGGAT
BKK05480 (mhqN::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTACGCTACACTCCCTTT,  downstream forward: _UP4_TATATGTAAAAGGAGCGGAT

LacZ fusion

BP1112: Promotor-lacZ fusion, available at Jörg Stülke's and Fabian Commichau's labs

References

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