meeF

168

general stress protein, manganese resistance protein

Locus: BSU_02920

Molecular weight: 29.02 kDa

Isoelectric point: 7.45

Protein length: 257 aa

Gene length: 774 bp

Function: resistance to Mn²⁺ intoxication, co-translocational metalation of Mn²⁺-dependent membrane and extracellular enzymes

Product: manganese resistance protein

Essential: no

Synonyms: yceF

Genomic Context

Hide small RNAsZoom:

Categories containing this gene/protein

Cellular processes, Homeostasis, Trace metal homeostasis (Cu, Zn, Ni, Mn, Mo), Manganese

Lifestyles, Coping with stress, General stress proteins (controlled by SigB)

Lifestyles, Coping with stress, Cell envelope stress proteins (controlled by SigM, V, W, X, Y)

Groups of genes, Membrane proteins

List of homologs in different organisms, belongs to COG0861 (Galperin et al., 2021)

This gene is a member of the following regulons

SigM regulon, SigW regulon, SigX regulon, SigB regulon

Gene

Coordinates: 314,025 → 314,798

Phenotypes of a mutant

the meeF meeY double mutant is defective in Protein secretion PubMed

The protein

Catalyzed reaction/ biological activity

co-translocational metalation of Mn²⁺-dependent membrane and extracellular enzymes PubMed

Protein family

TerC family PubMed

Structure

O34447 (AlphaFold)

Paralogous protein(s)

MeeY

Localization

cell membrane PubMed

Expression and Regulation

Operons

Genes: yceC-yceD-yceE-meeF-yceG-yceH

Description: PubMed

Regulation

induced by stress (SigB) PubMed

Sigma factors

SigM: sigma factor, PubMed, in sigM regulon
SigW: sigma factor, PubMed, in sigW regulon
SigB: sigma factor, PubMed, in sigB regulon
SigX: sigma factor, PubMed, in sigX regulon

Biological materials

Mutant

MGNA-B986 (yceF::erm), available at the NBRP B. subtilis, Japan
BKE02920 (ΔmeeF::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAGTTTTTTCCTCCTATAA,  downstream forward: _UP4_TAAAACAAAACAAGGAGGGA
BKK02920 (ΔmeeF::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAGTTTTTTCCTCCTATAA,  downstream forward: _UP4_TAAAACAAAACAAGGAGGGA

References

Reviews

Loading

Original Publications

Loading