pyrK

168

dihydroorotic acid dehydrogenase (electron transfer subunit)

Locus: BSU_15530

Molecular weight: 27.95 kDa

Isoelectric point: 5.58

Protein length: 256 aa

Gene length: 771 bp

Function: pyrimidine biosynthesis

Product: dihydroorotic acid dehydrogenase (electron transfer subunit)

Essential: no

Synonyms: pyrK, pyrDII, ylxD

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0543 (Galperin et al., 2021)

Gene

Coordinates: 1,626,948 1,627,718

The protein

Protein family

• PyrK family (single member, according to UniProt)

Domains

• FAD-binding FR-type domain (aa 1-101) (according to UniProt)

Cofactors

• [2Fe-2S] cluster PubMed
• FAD PubMed

Structure

• 1EP1 (PDB) (from Lactococcus lactis, 46% identity) PubMed
• P25983 (AlphaFold)

Additional information

• belongs to the 100 most abundant proteins PubMed

Expression and Regulation

Operons

• Genes: pyrR-pyrP-pyrB-pyrC-pyrAA-pyrAB-pyrK-pyrD-pyrF-pyrE

  Description: PubMed

  Regulation

  • induced in the absence of uridine nucleotides (PyrR) PubMed

  Regulatory mechanism

  • PyrR: termination, via RNA switch, in pyrR regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• BKE15530 (pyrK::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_ACATACTGTCAAATACGCTT, downstream forward: _UP4_AAAGCTCAGGAGGTGGCGCT
• BKK15530 (pyrK::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_ACATACTGTCAAATACGCTT, downstream forward: _UP4_AAAGCTCAGGAGGTGGCGCT

References

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