queA

168

S-adenosylmethionine tRNA ribosyltransferase

Locus: BSU_27720

Molecular weight: 38.35 kDa

Isoelectric point: 5.05

Protein length: 342 aa

Gene length: 1029 bp

Function: tRNA modification

Product: S-adenosylmethionine tRNA ribosyltransferase

Essential: no

Synonyms: queA

Genomic Context

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List of homologs in different organisms, belongs to COG0809 (Galperin et al., 2021)

Gene

Coordinates: 2,833,899  2,834,927

The protein

Catalyzed reaction/ biological activity

• 7-aminomethyl-7-carbaguanosine³⁴ in tRNA + S-adenosyl-L-methionine --> adenine + epoxyqueuosine³⁴ in tRNA + H⁺ + L-methionine (according to UniProt)

Protein family

• queA family (single member, according to UniProt)

Structure

• 1YY3 (PDB) PubMed
• O32054 (AlphaFold)

Expression and Regulation

Operons

• Genes: ruvA-ruvB-yrzS-queA-tgt-yrbF

  Description: PubMed

  Regulation

  • the mRNA is processed between tgt and yrbF by RNase Y, this requires the RicA-RicF-RicT complex PubMed

Biological materials

Mutant

• BKE27720 (queA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGATGATTTCACCTTTAT,  downstream forward: _UP4_TAATTTGAGAACAGGAGGCC
• BKK27720 (queA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGATGATTTCACCTTTAT,  downstream forward: _UP4_TAATTTGAGAACAGGAGGCC

References

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