sacA

168

phosphosucrase

Locus: BSU_38040

Molecular weight: 54.72 kDa

Isoelectric point: 5.34

Protein length: 480 aa

Gene length: 1440 bp

Function: utilization of sucrose

Product: phosphosucrase

Essential: no

E.C.: 3.2.1.26

Synonyms: sacA, ipa-50d

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG1621 (Galperin et al., 2021)

Gene

Coordinates: 3,902,210 → 3,903,649

The protein

Catalyzed reaction/ biological activity

• Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides (according to UniProt)

Protein family

• glycosyl hydrolase 32 family (with LevB and SacC, according to UniProt)

Structure

• 3PIG (PDB) (beta-fructofuranosidase from Bifidobacterium longum, 29% identity) PubMed
• P07819 (AlphaFold)

Expression and Regulation

Operons

• Genes: sacP-sacA-ywdA

  Description: PubMed

  Regulation

  • induced by sucrose (SacT) PubMed

  Regulatory mechanism

  • SacT: antitermination, via binding to a RNA switch, in sacT regulon
  • CcpA: repression, in ccpA regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• 1A810 ( sacA::cat), PubMed, available at BGSC
• 1A811 ( sacA::kan), PubMed, available at BGSC
• BKE38040 (ΔsacA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TGTCATTTTTTCCTCTCCTC,  downstream forward: _UP4_TAGAAAATCCTTCTATTTTC
• BKK38040 (ΔsacA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TGTCATTTTTTCCTCTCCTC,  downstream forward: _UP4_TAGAAAATCCTTCTATTTTC

References

Loading