cwlD

168

N-acetylmuramoyl-L-alanine amidase, spore cortex peptidoglycan synthesis

Locus: BSU_01530

Molecular weight: 26.86 kDa

Isoelectric point: 9.79

Protein length: 237 aa

Gene length: 714 bp

Function: spore cortex peptidoglycan synthesis

Product: N-acetylmuramoyl-L-alanine amidase

Essential: no

E.C.: 3.5.1.28

Synonyms: cwlD

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0860 (Galperin et al., 2021)

Gene

Coordinates: 156,612 → 157,325

The protein

Catalyzed reaction/ biological activity

• formation of muramic acid delta-lacton in spore cortex peptidoglycan PubMed
• Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides (according to UniProt)

Protein family

• N-acetylmuramoyl-L-alanine amidase 3 family (according to UniProt)

Domains

• contains an amidase_3 domain (like CwlC, LytC, YqiI, YrvJ)
• MurNAc-LAA domain (aa 43-226) (according to UniProt)

Structure

• 7RAG (PDB) (from Clostridium difficile, complex with GerS, 40.4% identity) PubMed
• P50864 (AlphaFold)

Localization

• secreted (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: ybaK-cwlD

  Description: PubMed

  Regulation

  • expressed during sporulation (SigG) PubMed

  Regulatory mechanism

  • LexA: repression, PubMed, in lexA regulon

  Sigma factors

  • SigE: sigma factor, PubMed, in sigE regulon
  • SigG: sigma factor, PubMed, in sigG regulon

• Genes: cwlD

  Description: PubMed

  Regulation

  • expressed during sporulation (SigG, SigE) PubMed

Biological materials

Mutant

• BKE01530 (ΔcwlD::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCCCTTCCCCTCCCGCTT,  downstream forward: _UP4_TAATGGAGGGTTTTCTTGTG
• BKK01530 (ΔcwlD::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCCCTTCCCCTCCCGCTT,  downstream forward: _UP4_TAATGGAGGGTTTTCTTGTG

References

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