epsK

168

export of extracellular poly-N-acetylglucosamine

Locus: BSU_34265

Molecular weight: 55.00 kDa

Isoelectric point: 10

Protein length: 505 aa

Gene length: 1518 bp

Function: export of extracellular poly-N-acetylglucosamine

Product: poly-N-acetylglucosamine exporter

Essential: no

Synonyms: epsK, yvfA, yvfB

Genomic Context

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List of homologs in different organisms, belongs to COG2244 (Galperin et al., 2021)

Gene

Coordinates: 3,517,485  3,519,002

Phenotypes of a mutant

• smooth colonies on MsGG medium, no biofilm formation PubMed

The EAR RNA switch

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The protein

Protein family

• MOP exporter family

Structure

• P71060 (AlphaFold)

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: epsA-epsB-epsC-epsD-epsE-epsF-epsG-epsH-epsI-epsJ-epsK-epsL-epsM-epsN-epsO

  Description: PubMed

  Regulation

  • repressed by SinR PubMed

  Regulatory mechanism

  • RemA: activation, PubMed, in remA regulon
  • AbrB: repression, PubMed, in abrB regulon
  • SinR: anti-activation, (prevents binding of RemA) PubMed, in sinR regulon
  • EAR riboswitch: processive antitermination, in EAR riboswitch

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

  Additional information

  • induction by sequestration of SinR by SinI or SlrA PubMed

Biological materials

Mutant

• MGNA-B611 (yvfA::erm), available at the NBRP B. subtilis, Japan
• BKE34265 (epsK::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CGCGCTGAAATTTATCGTGA,  downstream forward: _UP4_AAAACGAAAGGAGCTGTGAA
• BKK34265 (epsK::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CGCGCTGAAATTTATCGTGA,  downstream forward: _UP4_AAAACGAAAGGAGCTGTGAA

Labs working on this gene/protein

• Richard Losick, Harvard Univ., Cambridge, USA homepage

References

Reviews

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