yhaM

168

3′-deoxyribonuclease specific for ssDNA

Locus: BSU_09930

Molecular weight: 35.53 kDa

Isoelectric point: 5.85

Protein length: 314 aa

Gene length: 945 bp

Function: uptake and processing of ssDNA

Product: 3′-deoxyribonuclease

Essential: no

Synonyms: yhaM

Genomic Context

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Categories containing this gene/protein

Information processing, Genetics, Genetic competence

List of homologs in different organisms, belongs to COG3481 (Galperin et al., 2021)

This gene is a member of the following regulons

LexA regulon

Gene

Coordinates: 1,069,042  1,069,986

Phenotypes of a mutant

transformation by plasmid and genomic DNA is strongly impaired PubMed

The protein

Catalyzed reaction/ biological activity

degradation of incoming ssDNA PubMed

Protein family

YhaM family (single member, according to UniProt)

Domains

HD domain (aa 163-297) (according to UniProt)

Structure

9H3F (PDB) PubMed
O07521 (AlphaFold)

Localization

cytoplasm, with formation of foci PubMed

Additional information

subject to Clp-dependent proteolysis upon glucose starvation PubMed

Expression and Regulation

Operons

Genes: yhaO-sbcE-yhaM

Description: PubMed

Regulation

induced by DNA damage (LexA) PubMed

Regulatory mechanism

LexA: repression, PubMed, in lexA regulon

Biological materials

Mutant

MGNA-B499 (yhaM::erm), available at the NBRP B. subtilis, Japan
BKE09930 (yhaM::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCTATTAAAGCTCCCTC,  downstream forward: _UP4_TAACTTAAAAAGGACTGATG
BKK09930 (yhaM::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCTATTAAAGCTCCCTC,  downstream forward: _UP4_TAACTTAAAAAGGACTGATG

Labs working on this gene/protein

David Bechhofer, Mount Sinai School, New York, USA Laboratories and Programs/Bechhofer Laboratory?citype=Physician&ciid=Bechhofer David H 1255565 Homepage

References

Reviews

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Original Publications

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