xylA

168

xylose isomerase

Locus: BSU_17600

Molecular weight: 50.09 kDa

Isoelectric point: 5.64

Protein length: 445 aa

Gene length: 1338 bp

Function: utilization of xylan and xylose

Product: xylose isomerase

Essential: no

E.C.: 5.3.1.5

Synonyms: xylA

Genomic Context

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List of homologs in different organisms, belongs to COG2115 (Galperin et al., 2021)

Gene

Coordinates: 1,891,908  1,893,245

The protein

Catalyzed reaction/ biological activity

• D-xylose --> D-xylulose

Protein family

• xylose isomerase family (according to Swiss-Prot)

Structure

• 1A0D (PDB) (Geobacillus stearothermophilus)

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: xylA-xylB

  Description: PubMed

  Regulation

  • carbon catabolite repression (CcpA) PubMed

  Regulatory mechanism

  • CcpA: repression, PubMed, in ccpA regulon
  • XylR: repression, PubMed, in xylR regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• GP1151 (del aphA3) available in Jörg Stülke's lab
• 1A719 (no resistance), PubMed, available at BGSC
• BKE17600 (xylA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTGATTTCCCCCTTAAA,  downstream forward: _UP4_TAACAGGATAAGCTCCAGAT
• BKK17600 (xylA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTGATTTCCCCCTTAAA,  downstream forward: _UP4_TAACAGGATAAGCTCCAGAT

References

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