mifM

168

ribosome-nascent chain sensor of membrane protein biogenesis

Locus: BSU_23880

Molecular weight: 11.01 kDa

Isoelectric point: 5.83

Protein length: 95 aa

Gene length: 288 bp

Function: control of YidC2 translation

Product: sensor of SpoIIIJ activity

Essential: no

Synonyms: mifM, yqzJ

Genomic Context

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List of homologs in different organisms

Gene

Coordinates: 2,483,586 → 2,483,873

The protein

Catalyzed reaction/ biological activity

• acts as a kind of "leader peptide" that does or does not allow translation through a hairpin structure in the ''yidC2'' mRNA PubMed

Domains

• N-terminal transmembrane domain PubMed
• the C-terminal domain interacts with the ribosome and arrests the own translation elongation PubMed

Structure

• 3J9W (PDB) (MifM-stalled ribosome complex) PubMed
• EMD-6306 (MifM-stalled ribosome complex) PubMed
• Q7WY64 (AlphaFold)

Localization

• membrane PubMed

Expression and Regulation

Operons

• Genes: mifM-yidC2

  Description: PubMed

  Regulation

  • repressed by large amounts of SpoIIIJ, induced by small amounts of SpoIIIJ (MifM) PubMed

  Regulatory mechanism

  • MifM: attenuation, an mRNA hairpin of the yqjG transcript unfold upon translation arrest of MifM at decreased YidC1 levels, in mifM regulon

  Additional information

  • An antisense RNA is predicted for 'mifM' PubMed

Biological materials

Mutant

• BKE23880 (ΔmifM::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCACATCCATTCAATAGC,  downstream forward: _UP4_TAAACCGCATTTATAAAAAG
• BKK23880 (ΔmifM::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCACATCCATTCAATAGC,  downstream forward: _UP4_TAAACCGCATTTATAAAAAG

References

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