drm

168

phosphopentomutase

Locus: BSU_23500

Molecular weight: 43.77 kDa

Isoelectric point: 4.97

Protein length: 394 aa

Gene length: 1185 bp

Function: utilization of deoxyribose

Product: phosphopentomutase

Essential: no

E.C.: 5.4.2.7

Synonyms: drm, yqkN

Genomic Context

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List of homologs in different organisms, belongs to COG1015 (Galperin et al., 2021)

Gene

Coordinates: 2,447,246  2,448,430

The protein

Catalyzed reaction/ biological activity

• α-D-ribose 1-phosphate --> D-ribose 5-phosphate (according to UniProt)
• 2-deoxy-α-D-ribose 1-phosphate --> 2-deoxy-D-ribose 5-phosphate (according to UniProt)

Protein family

• phosphopentomutase family (single member, according to UniProt)

Structure

• 3M8W (PDB) (from B. cereus, 77% identity, 86% similarity) PubMed
• P46353 (AlphaFold)

Modification

• phosphorylation on (Thr-87 OR Thr-89) PubMed
• CoA is attached to Cys-233 in spores PubMed

Expression and Regulation

Operons

• Genes: drm-pupG

  Description: PubMed

  Regulation

  • induced in the presence of nucleosides (deoxyribose 5-phosphate and ribose 5-phosphate act as molecular inducers) PubMed

  Regulatory mechanism

  • CcpA: repression, PubMed, in ccpA regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• BKE23500 (drm::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTTGAAAGCCTCCTTTT,  downstream forward: _UP4_TAGGGGGACTGTTTCTTGAA
• BKK23500 (drm::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTTGAAAGCCTCCTTTT,  downstream forward: _UP4_TAGGGGGACTGTTTCTTGAA

References

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