frlN

168

aminosugar ABC transporter (permease)

Locus: BSU_32590

Molecular weight: 32.68 kDa

Isoelectric point: 5.29

Protein length: 292 aa

Gene length: 879 bp

Function: uptake of sugar amines

Product: aminosugar ABC transporter (permease)

Essential: no

Synonyms: frlN, yurN

Genomic Context

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List of homologs in different organisms, belongs to COG1175 (Galperin et al., 2021)

Gene

Coordinates: 3,348,825  3,349,703

The protein

Protein family

• Binding-protein-dependent transport system permease family (according to UniProt)
• MalFG subfamily (according to UniProt)

Domains

• ABC transmembrane type-1 domain (aa 66-282) (according to UniProt)

Structure

• 4TQU (PDB) (from Sphingomonas sp., 28% identity) PubMed
• O32155 (AlphaFold)

Localization

• cell membrane PubMed

Expression and Regulation

Operons

• Genes: frlB-frlO-frlN-frlM-frlD-frlP

  Description: PubMed

  Regulation

  • '' FrlB'': repressed during growth in the presence of branched chain amino acids (CodY) PubMed
  • induced in the absence of RnpM PubMed

  Regulatory mechanism

  • CodY: repression, PubMed, PubMed, in codY regulon
  • FrlR: repression, PubMed, in frlR regulon
  • RnpM: unknown, PubMed, in rnpM regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

  Additional information

  • the frlB-frlO-frlN-frlM-frlD-frlP operon is strongly downregulated in a cshA mutant PubMed

Biological materials

Mutant

• MGNA-A560 (yurN::erm), available at the NBRP B. subtilis, Japan
• BKE32590 (frlN::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAGGGTATCTCATCTCCTT,  downstream forward: _UP4_AAAACCGGAAAGGAGGAGTA
• BKK32590 (frlN::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAGGGTATCTCATCTCCTT,  downstream forward: _UP4_AAAACCGGAAAGGAGGAGTA

References

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