yxjM

168

two-component sensor kinase

Locus: BSU_38900

Molecular weight: 45.07 kDa

Isoelectric point: 6.8

Protein length: 406 aa

Gene length: 1221 bp

Function: unknown

Product: two-component sensor kinase (NarL family)

Essential: no

Synonyms: yxjM

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG4585 (Galperin et al., 2021)

Gene

Coordinates: 3,993,162  3,994,382

The protein

Catalyzed reaction/ biological activity

• autophosphorylation, phosphorylation of YxjL
• ATP + protein L-histidine --> ADP + protein N-phospho-L-histidine (according to UniProt)

Domains

• Histidine kinase domain (aa 209-396) (according to UniProt)

Structure

• P55183 (AlphaFold)

Modification

• autophosphorylation on a His residue

Localization

• cell membrane (according to UniProt)

Biological materials

Mutant

• MGNA-B801 (yxjM::erm), available at the NBRP B. subtilis, Japan
• BKE38900 (yxjM::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GTTCATGCTGGTCCTTCTTT,  downstream forward: _UP4_ATGACAACGAATAAGGAGCA
• BKK38900 (yxjM::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GTTCATGCTGGTCCTTCTTT,  downstream forward: _UP4_ATGACAACGAATAAGGAGCA

References

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