fliS

168

chaperone for the export of Flagellin

Locus: BSU_35330

Molecular weight: 14.99 kDa

Isoelectric point: 5.23

Protein length: 133 aa

Gene length: 402 bp

Function: Motility and chemotaxis

Product: chaperone for Flagellin export

Essential: no

Synonyms: fliS

Genomic Context

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Categories containing this gene/protein

Lifestyles, Exponential and early post-exponential lifestyles, Motility and chemotaxis, Flagellar proteins

Lifestyles, Exponential and early post-exponential lifestyles, Swarming

List of homologs in different organisms, belongs to COG1516 (Galperin et al., 2021)

This gene is a member of the following regulons

SigD regulon

Gene

Coordinates: 3,632,488  3,632,889

Phenotypes of a mutant

defective in Swarming motility PubMed

The protein

Catalyzed reaction/ biological activity

prevents premature assembly of Hag PubMed

Protein family

FliS family (single member, according to UniProt)

Structure

1VH6 (PDB) PubMed
6GOW (PDB) (complex with flagellin) PubMed
P39739 (AlphaFold)

Localization

cytoplasm (according to UniProt)

Expression and Regulation

Operons

Genes: yvyC-fliD-fliS-fliT-smiA-S1350-hpf

Description: PubMed

Sigma factors

SigD: sigma factor, PubMed, in sigD regulon

Genes: yvyC-fliD-fliS-fliT

Description: PubMed

Sigma factors

SigD: sigma factor, PubMed, in sigD regulon

Biological materials

Mutant

BKE35330 (fliS::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTGTCATCCTCCAAATT,  downstream forward: _UP4_CGGCACGGATCAGGCGGGAT
BKK35330 (fliS::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTGTCATCCTCCAAATT,  downstream forward: _UP4_CGGCACGGATCAGGCGGGAT

References

Reviews

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Original Publications

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