fadM

168

fatty acid degradation, similar to proline dehydrogenase

Locus: BSU_32850

Molecular weight: 34.30 kDa

Isoelectric point: 9.38

Protein length: 302 aa

Gene length: 909 bp

Function: fatty acid degradation

Product: unknown

Essential: no

Synonyms: fadM, yusM

Genomic Context

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Categories containing this gene/protein

Metabolism, Lipid metabolism, Utilization of lipids, Utilization of fatty acids

Lifestyles, Sporulation, Sporulation proteins, Newly identified sporulation proteins (based on transcription profiling)

List of homologs in different organisms, belongs to COG0506 (Galperin et al., 2021)

Gene

Coordinates: 3,372,740 → 3,373,648

The protein

Catalyzed reaction/ biological activity

quinone + L-proline --> (S)-1-pyrroline-5-carboxylate + quinol + H⁺ (according to UniProt)

Protein family

proline dehydrogenase family (with PutB, according to UniProt)

Cofactors

FAD (according to UniProt)

Structure

4H6Q (PDB) (from Deinococcus radiodurans, 48% identity) PubMed
O32179 (AlphaFold)

Paralogous protein(s)

PutB

Expression and Regulation

Operons

Genes: fadM-fadN-fadA-fadE

Description: PubMed

Regulation

''fadM'': induced by long-chain fatty acids (FadR) PubMed

Regulatory mechanism

FadR: repression, PubMed, in fadR regulon
SdpR: activation, PubMed, in sdpR regulon

Biological materials

Mutant

MGNA-A598 (yusM::erm), available at the NBRP B. subtilis, Japan
BKE32850 (ΔfadM::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCCGACTCCCTCCCGCCG,  downstream forward: _UP4_TGAAAGCAGCCGTGGGAAGC
BKK32850 (ΔfadM::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCCGACTCCCTCCCGCCG,  downstream forward: _UP4_TGAAAGCAGCCGTGGGAAGC

References

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