Difference between revisions of "CzrA"

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* '''Description:''' transcriptional repressor of ''[[cadA]]'' and ''[[czcD]]'' <br/><br/>
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* '''Description:''' transcriptional repressor ([[transcription factors of the ArsR family|ArsR family]]), ''[[cadA]]'' and ''[[czcD]]'' <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
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|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
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|style="background:#ABCDEF;" align="center"| '''Product''' || transcriptional repressor (ArsR family)  
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|style="background:#ABCDEF;" align="center"| '''Product''' || transcriptional repressor ([[transcription factors of the ArsR family|ArsR family]])  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Function''' || regulation of resistance against toxic metal cations
 
|style="background:#ABCDEF;" align="center"|'''Function''' || regulation of resistance against toxic metal cations
 +
|-
 +
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU19120 czrA]
 +
|-
 +
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://cellpublisher.gobics.de/view/GqbblRJkBH metal ion homeostasis]'''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 12 kDa, 5.719   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 12 kDa, 5.719   
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yobW]]'', ''[[yocA]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yobW]]'', ''[[yocA]]''
 
|-
 
|-
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB13804&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
+
|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU19120 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU19120 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU19120 DNA_with_flanks]
 
|-
 
|-
 
|colspan="2" | '''Genetic context''' <br/> [[Image:yozA_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:yozA_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 +
|-
 +
|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=czrA_2084786_2085109_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:czrA_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU19120]]
 
|-
 
|-
 
|}
 
|}
  
 
__TOC__
 
__TOC__
−
 
+
<br/><br/><br/><br/>
 +
<br/><br/><br/><br/>
 +
<br/><br/><br/><br/>
 
<br/><br/>
 
<br/><br/>
  
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= This gene is a member of the following [[regulons]] =
 
= This gene is a member of the following [[regulons]] =
−
 
+
=The [[CzrA regulon]]:=
 +
* ''[[cadA]]'', ''[[czcD]]-[[trkA]]''
  
 
=The gene=
 
=The gene=
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=== Database entries ===
 
=== Database entries ===
 +
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU19120&redirect=T BSU19120]
  
 
* '''DBTBS entry:''' no entry
 
* '''DBTBS entry:''' no entry
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=== Additional information===
 
=== Additional information===
−
 
−
 
−
 
  
 
=The protein=
 
=The protein=
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* '''Catalyzed reaction/ biological activity:'''  
 
* '''Catalyzed reaction/ biological activity:'''  
  
−
* '''Protein family:'''
+
* '''Protein family:''' [[transcription factors of the ArsR family|ArsR family]]
  
 
* '''Paralogous protein(s):'''
 
* '''Paralogous protein(s):'''
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** DNA-binding activity of CzrA is inactivated by high concentrations of toxic metal ions (induction)
 
** DNA-binding activity of CzrA is inactivated by high concentrations of toxic metal ions (induction)
  
−
* '''Interactions:'''
+
* '''[[SubtInteract|Interactions]]:'''
  
−
* '''Localization:'''
+
* '''[[Localization]]:'''
  
 
=== Database entries ===
 
=== Database entries ===
 +
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU19120&redirect=T BSU19120]
  
 
* '''Structure:'''
 
* '''Structure:'''
−
 
+
** [http://www.rcsb.org/pdb/explore/explore.do?structureId=1R1U 1R1U] (the apo-repressor from ''Staph. aureus'', 49% identity, 82% similarity) {{PubMed|14568530}}
 +
** [http://www.rcsb.org/pdb/explore/explore.do?structureId=1R1V 1R1V] (the Zn(II) form from ''Staph. aureus'', 49% identity, 82% similarity) {{PubMed|14568530}}
 +
** [http://www.rcsb.org/pdb/explore/explore.do?structureId=2KJB 2KJB] (the DNA-bound form from ''Staph. aureus'', 49% identity, 82% similarity) {{PubMed|19822742}}
 
* '''UniProt:''' [http://www.uniprot.org/uniprot/O31844 O31844]
 
* '''UniProt:''' [http://www.uniprot.org/uniprot/O31844 O31844]
  
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* '''Operon:'''  
 
* '''Operon:'''  
  
−
* '''[[Sigma factor]]:'''  
+
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=czrA_2084786_2085109_-1 czrA] {{PubMed|22383849}}
 +
 
 +
* '''Sigma factor:'''  
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
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* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
  
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* '''Additional information:'''  
+
* '''Additional information:'''
  
 
=Biological materials =
 
=Biological materials =
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=References=
 
=References=
−
 
+
<pubmed>15948947, 16430705, 14568530 19822742 25213752 22007899</pubmed>
−
<pubmed>15948947,,16430705, </pubmed>
 
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Latest revision as of 09:24, 19 September 2014

Gene name czrA
Synonyms yozA
Essential no
Product transcriptional repressor (ArsR family)
Function regulation of resistance against toxic metal cations
Gene expression levels in SubtiExpress: czrA
Metabolic function and regulation of this protein in SubtiPathways:
metal ion homeostasis
MW, pI 12 kDa, 5.719
Gene length, protein length 321 bp, 107 aa
Immediate neighbours yobW, yocA
Sequences Protein DNA DNA_with_flanks
Genetic context
YozA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
CzrA expression.png















Categories containing this gene/protein

trace metal homeostasis (Cu, Zn, Ni, Mn, Mo), transcription factors and their control, resistance against toxic metals

This gene is a member of the following regulons

The CzrA regulon:

The gene

Basic information

  • Locus tag: BSU19120

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
    • DNA-binding activity of CzrA is inactivated by high concentrations of toxic metal ions (induction)

Database entries

  • Structure:
    • 1R1U (the apo-repressor from Staph. aureus, 49% identity, 82% similarity) PubMed
    • 1R1V (the Zn(II) form from Staph. aureus, 49% identity, 82% similarity) PubMed
    • 2KJB (the DNA-bound form from Staph. aureus, 49% identity, 82% similarity) PubMed
  • UniProt: O31844
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Zhen Ma, Pete Chandrangsu, Tyler C Helmann, Adisak Romsang, Ahmed Gaballa, John D Helmann
Bacillithiol is a major buffer of the labile zinc pool in Bacillus subtilis.
Mol Microbiol: 2014, 94(4);756-70
[PubMed:25213752] [WorldCat.org] [DOI] (I p)

Dhruva K Chakravorty, Bing Wang, Chul Won Lee, David P Giedroc, Kenneth M Merz
Simulations of allosteric motions in the zinc sensor CzrA.
J Am Chem Soc: 2012, 134(7);3367-76
[PubMed:22007899] [WorldCat.org] [DOI] (I p)

Alphonse I Arunkumar, Gregory C Campanello, David P Giedroc
Solution structure of a paradigm ArsR family zinc sensor in the DNA-bound state.
Proc Natl Acad Sci U S A: 2009, 106(43);18177-82
[PubMed:19822742] [WorldCat.org] [DOI] (I p)

Duncan R Harvie, Claudia Andreini, Gabriele Cavallaro, Wenmao Meng, Bernard A Connolly, Ken-ichi Yoshida, Yasutaro Fujita, Colin R Harwood, David S Radford, Stephen Tottey, Jennifer S Cavet, Nigel J Robinson
Predicting metals sensed by ArsR-SmtB repressors: allosteric interference by a non-effector metal.
Mol Microbiol: 2006, 59(4);1341-56
[PubMed:16430705] [WorldCat.org] [DOI] (P p)

Charles M Moore, Ahmed Gaballa, Monica Hui, Rick W Ye, John D Helmann
Genetic and physiological responses of Bacillus subtilis to metal ion stress.
Mol Microbiol: 2005, 57(1);27-40
[PubMed:15948947] [WorldCat.org] [DOI] (P p)

Christoph Eicken, Mario A Pennella, Xiaohua Chen, Karl M Koshlap, Michael L VanZile, James C Sacchettini, David P Giedroc
A metal-ligand-mediated intersubunit allosteric switch in related SmtB/ArsR zinc sensor proteins.
J Mol Biol: 2003, 333(4);683-95
[PubMed:14568530] [WorldCat.org] [DOI] (P p)

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