rplGA

168

similar to ribosomal protein, L7AE family, binds K-turns in RNA switches

Locus: BSU_16620

Molecular weight: 10.92 kDa

Isoelectric point: 10.17

Protein length: 100 aa

Gene length: 303 bp

Function: unknown

Product: unknown

Essential: no

Synonyms: rplGA, ylxQ, ymxC, rpmXA, ktuQ

Genomic Context

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List of homologs in different organisms, belongs to COG1358 (Galperin et al., 2021)

Gene

Coordinates: 1,733,687  1,733,989

The protein

Catalyzed reaction/ biological activity

• binds K-turns in RNA switches as the occur in the L-box, the S-box and the T-box PubMed

Protein family

• Eukaryotic ribosomal protein eL8 family (with RplGB, according to UniProt)

Structure

• 3V7Q (PDB) PubMed
• P32729 (AlphaFold)

Localization

• ribosome (according to UniProt)

Expression and Regulation

Operons

• Genes: ylxS-nusA-rnpM-rplGA-infB-ylxP-rbfA

  Description: PubMed

  Regulation

  • autoregulation NusA PubMed
  • induced by glucose PubMed
  • expression is reduced in motile cells as compared to non-motile cells PubMed

  Regulatory mechanism

  • stringent response: negative regulation, in stringent response
  • NusA: attenuation, NusA stimulates termination PubMed, in nusA regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon
  • SigB: sigma factor, PubMed, in sigB regulon

Biological materials

Mutant

• BKE16620 (rplGA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GGGAAACCATTCCATTCCAG,  downstream forward: _UP4_AATAAGCTGATCAGCTTGCTCG
• BKK16620 (rplGA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GGGAAACCATTCCATTCCAG,  downstream forward: _UP4_AATAAGCTGATCAGCTTGCTCG

References

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