ctaO

168

heme O synthase (minor enzyme)

Locus: BSU_12080

Molecular weight: 36.73 kDa

Isoelectric point: 10.12

Protein length: 329 aa

Gene length: 990 bp

Function: heme biosynthesis

Product: heme O synthase (minor enzyme)

Essential: no

E.C.: null

Synonyms: ctaO, yjdK

Genomic Context

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List of homologs in different organisms, belongs to COG0109 (Galperin et al., 2021)

Gene

Coordinates: 1,279,514  1,280,503

The protein

Catalyzed reaction/ biological activity

• (2E,6E)-farnesyl diphosphate + H₂O + heme b --> diphosphate + Fe(II)-heme o (according to UniProt)

Protein family

• UbiA prenyltransferase family (with CtaB, according to UniProt)

Structure

• O31652 (AlphaFold)

Paralogous protein(s)

• CtaB

Localization

• cell membrane (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: ctaO

  Description: PubMed

  Regulatory mechanism

  • AbrB: repression, PubMed, in abrB regulon

  Additional information

  • the amount of the mRNA is substantially decreased upon depletion of RNase Y PubMed

Biological materials

Mutant

• MGNA-A273 (yjdK::erm), available at the NBRP B. subtilis, Japan
• BKE12080 (ctaO::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGCTTTTGTTATCGTAAC,  downstream forward: _UP4_TGATGCAAAAAGAGCCACAC
• BKK12080 (ctaO::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGCTTTTGTTATCGTAAC,  downstream forward: _UP4_TGATGCAAAAAGAGCCACAC

References

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