purL

168

phosphoribosylformylglycinamidine synthase

locus: BSU_06480

Molecular weight: 80.10 kDa

pI: 4.55

Protein length: 742 aa

Gene length: 2229 bp

function: purine biosynthesis

product: phosphoribosylformylglycinamidine synthase

essential: no

ec: 6.3.5.3

synonyms: purL

List of homologs in different organisms, belongs to COG0046 (Galperin et al., 2021)

Gene

Coordinates: 703,237 705,465

The protein

Catalyzed reaction/ biological activity

• ATP + H₂O + L-glutamine + N²-formyl-N¹-(5-phospho-D-ribosyl)glycinamide --> 2-(formamido)-N¹-(5-phospho-D-ribosyl)acetamidine + ADP + H⁺ + L-glutamate + phosphate(according to UniProt)
• H₂O + L-glutamine --> L-glutamate + NH₄⁺ (according to UniProt)

Protein family

• FGAMS family (single member, according to UniProt)

Structure

• [PDB|3D54] (from ''Thermotoga maritima'', 39% identity, 58% similarity) [Pubmed|18597481]
• [AF|P12042]

[wiki|Localization]

• cytoplasm (according to UniProt)
• membrane associated [Pubmed|18763711]

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation [PubMed|17981983]
• belongs to the 100 most abundant proteins [PubMed|15378759]

Expression and Regulation

Operons

• genes: [gene|1A9F7847AA1EB2A264842B44CD1B1A21F0AF6E57|purE]-[gene|0DA73E4D1935F6A91BE251EF2704A11DE5D81174|purK]-[gene|29FF54E2B9F4A11811F5E15885A4C02A259C7B35|purB]-[gene|447D12E940C7F45B6C5015844F9DED9D513CD6E5|purC]-[gene|CCD4DA969086181CFA33D6D241F2CAAE02E0C758|purS]-[gene|DEE856E83E790EB57F103F7A9156408AF090995D|purQ]-[gene|D34203FAA84C19B34B3CB5F00AC2E2663FFA344D|purL]-[gene|4AA5AF9C7CE8D0A9FD3A47650ECF130BCBEE1952|purF]-[gene|AD0DE9457D70DF02FA0F58867B24ABF236FAF65A|purM]-[gene|7B30F27D6C459437967A64467E52961F4A9E0F2C|purN]-[gene|54CE8EB2F5D1F9AEAA497E1F60248C9B2250B01E|purH]-[gene|9BBA22B09C6DD6C36E043D44B0F54BD0FFA5BA0E|purD]

  description: [Pubmed|3036807]

  regulation

  • expression activated by glucose (4.4 fold) [Pubmed|12850135]

  regulatory mechanism

  • G-box: RNA switch, ([wiki|riboswitch]) [pubmed|3036807,12787499], in [regulon|other_regulator:G-box|G-box]
  • [protein|A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C|purR]: repression, (molecular inducer: PRPP) [Pubmed|7638212,2536750], in [regulon|protein:A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C|purR regulon]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|3036807], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

Biological materials

Mutant

• BKE06480 ([gene|D34203FAA84C19B34B3CB5F00AC2E2663FFA344D|purL]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE06480 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GTTCAAGCAGTAGTGACATG, downstream forward: _UP4_CCATGCTTGCTGAAATCAAA
• BKK06480 ([gene|D34203FAA84C19B34B3CB5F00AC2E2663FFA344D|purL]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK06480 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GTTCAAGCAGTAGTGACATG, downstream forward: _UP4_CCATGCTTGCTGAAATCAAA

References

• 15301530,3036807,12923093,21671997,7638212,18763711,15378759