purL

168

phosphoribosylformylglycinamidine synthase

Locus: BSU_06480

Molecular weight: 80.10 kDa

Isoelectric point: 4.55

Protein length: 742 aa

Gene length: 2229 bp

Function: purine biosynthesis

Product: phosphoribosylformylglycinamidine synthase

Essential: no

E.C.: 6.3.5.3

Synonyms: purL

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0046 (Galperin et al., 2021)

Gene

Coordinates: 703,237 705,465

The protein

Catalyzed reaction/ biological activity

• ATP + H₂O + L-glutamine + N²-formyl-N¹-(5-phospho-D-ribosyl)glycinamide --> 2-(formamido)-N¹-(5-phospho-D-ribosyl)acetamidine + ADP + H⁺ + L-glutamate + phosphate(according to UniProt)
• H₂O + L-glutamine --> L-glutamate + NH₄⁺ (according to UniProt)

Protein family

• FGAMS family (single member, according to UniProt)

Structure

• 3D54 (PDB) (from Thermotoga maritima, 39% identity, 58% similarity) PubMed
• P12042 (AlphaFold)

Localization

• cytoplasm (according to UniProt)
• membrane associated PubMed

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation PubMed
• belongs to the 100 most abundant proteins PubMed

Expression and Regulation

Operons

• Genes: purE-purK-purB-purC-purS-purQ-purL-purF-purM-purN-purH-purD

  Description: PubMed

  Regulation

  • expression activated by glucose (4.4 fold) PubMed

  Regulatory mechanism

  • G-box: RNA switch, (riboswitch) PubMed, in G-box
  • PurR: repression, (molecular inducer: PRPP) PubMed, in purR regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• BKE06480 (purL::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_GTTCAAGCAGTAGTGACATG, downstream forward: _UP4_CCATGCTTGCTGAAATCAAA
• BKK06480 (purL::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_GTTCAAGCAGTAGTGACATG, downstream forward: _UP4_CCATGCTTGCTGAAATCAAA

References

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