purL

purL
168

phosphoribosylformylglycinamidine synthase

Locus
BSU_06480
Molecular weight
80.10 kDa
Isoelectric point
4.55
Protein length
Gene length
Function
purine biosynthesis
Product
phosphoribosylformylglycinamidine synthase
Essential
no
E.C.
6.3.5.3
Synonyms
purL

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0046 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
703,237 705,465
The protein
Catalyzed reaction/ biological activity
ATP + H2O + L-glutamine + N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide --> 2-(formamido)-N1-(5-phospho-D-ribosyl)acetamidine + ADP + H+ + L-glutamate + phosphate(according to UniProt)
H2O + L-glutamine --> L-glutamate + NH4+ (according to UniProt)
Protein family
FGAMS family (single member, according to UniProt)
Structure
3D54 (PDB) (from Thermotoga maritima, 39% identity, 58% similarity) PubMed
cytoplasm (according to UniProt)
membrane associated PubMed
Additional information
subject to Clp-dependent proteolysis upon glucose starvation PubMed
belongs to the 100 most abundant proteins PubMed
Expression and Regulation
Operons
Description
Regulation
expression activated by glucose (4.4 fold) PubMed
Regulatory mechanism
G-box: RNA switch, (riboswitch) PubMed, in G-box
PurR: repression, (molecular inducer: PRPP) PubMed, in purR regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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purEpurD

2025-08-18 14:49:59

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Biological materials
Mutant
BKE06480 (purL::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_GTTCAAGCAGTAGTGACATG, downstream forward: _UP4_CCATGCTTGCTGAAATCAAA
BKK06480 (purL::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_GTTCAAGCAGTAGTGACATG, downstream forward: _UP4_CCATGCTTGCTGAAATCAAA
References
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Page visits: 5160

Time of last update: 2025-08-18 19:31:36

Author of last update: Melvin.boenninger