yaaK

168

similar to DNA-binding protein

Locus: BSU_00200

Molecular weight: 11.64 kDa

Isoelectric point: 4.54

Protein length: 107 aa

Gene length: 324 bp

Function: unknown

Product: unknown

Essential: no

Synonyms: yaaK

Genomic Context

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List of homologs in different organisms, belongs to COG0718 (Galperin et al., 2021)

Gene

Coordinates: 28,529  28,852

The protein

Protein family

• YbaB/EbfC family (single member, according to UniProt)

Structure

• 1YBX (PDB) (from Clostridium thermocellum, 53% identity)
• P24281 (AlphaFold)

Localization

• nucleoid (according to UniProt)

Expression and Regulation

Operons

• Genes: dnaX-yaaK-recR-yaaL-bofA

  Description: PubMed

Additional information

• the mRNA is very stable (> 15 min) PubMed

Biological materials

Mutant

• MGNA-B892 (yaaK::erm), available at the NBRP B. subtilis, Japan
• BKE00200 (yaaK::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAGCATTCACTCTCTTTC,  downstream forward: _UP4_TTATTCTAGGGGGATAAAAG
• BKK00200 (yaaK::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAGCATTCACTCTCTTTC,  downstream forward: _UP4_TTATTCTAGGGGGATAAAAG

References

Reviews

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Original Publications

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