ywrD

ywrD
168

unknown, similar to gamma-glutamyltransferase (Ggt)

Locus
BSU_36100
Molecular weight
57.32 kDa
Isoelectric point
5.45
Protein length
Gene length
Function
unknown
Product
unknown
Essential
no
E.C.
2.3.2.2
Synonyms
ywrD

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0405 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,719,134 → 3,720,711
The protein
Catalyzed reaction/ biological activity
an α-amino acid + an N-terminal (5-L-glutamyl)-[peptide] --> 5-L-glutamyl amino acid + N-terminal L-α-aminoacyl-[peptide] (according to UniProt)
glutathione + H2O --> L-cysteinylglycine + L-glutamate (according to UniProt)
an S-substituted glutathione + H2O --> an S-substitued L-cysteinylglycine + L-glutamate, however, all evidence suggests this is a misannotation PubMed
Protein family
gamma-glutamyltransferase family (with Ggt, according to UniProt), however, all evidence suggests this is a misannotation PubMed
Structure
2NLZ (PDB) (from B. halodurans, 38% identity)
Additional information
The gene is annotated in KEGG as an ortholog of gamma-glutamyltranspeptidase EC 2.3.2.2, This enzyme is necessary to utilize glutathione (GSH) as the sulfur source. No EC annotation for this gene is available in Swiss-Prot/MetaCyc. It has been shown by Minami et al. (PubMed) that ywrD mutant grows well on minimal media supplied with GSH as the sole sulfur source. In addition, His-tag purified ywrD cannot hydrolyze GSH. PubMed
Expression and Regulation
Operons
Genes
Description
Regulation
expressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
Regulatory mechanism
TnrA: activation, PubMed, in tnrA regulon
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ywrD

2025-01-14 17:58:41

ghost

44

173eba87f43e5b4d394e6ffa911740216f2f0599

FAFAE581ACB24823A158FF184C44DA48157D8CEE

Biological materials
Mutant
MGNA-B653 (ywrD::erm), available at the NBRP B. subtilis, Japan
BKE36100 (ΔywrD::erm  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CATCACAAGTCCCCTTTTTT,  downstream forward: _UP4_GGAGCGGCTGTGGGGATTTA
BKK36100 (ΔywrD::kan  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CATCACAAGTCCCCTTTTTT,  downstream forward: _UP4_GGAGCGGCTGTGGGGATTTA
References
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F1DF59528E340A65A982BD7E6621DDA82C10B2B5

Page visits: 2523

Time of last update: 2025-01-14 23:39:02

Author of last update: Melvin.boenninger