ywrD
168
unknown, similar to gamma-glutamyltransferase (Ggt)
Locus
BSU_36100
Molecular weight
57.32 kDa
Isoelectric point
5.45
Function
unknown
Product
unknown
Essential
no
E.C.
2.3.2.2
Synonyms
ywrD
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0405 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,719,134 → 3,720,711
The protein
Catalyzed reaction/ biological activity
an α-amino acid + an N-terminal (5-L-glutamyl)-[peptide] --> 5-L-glutamyl amino acid + N-terminal L-α-aminoacyl-[peptide] (according to UniProt)
glutathione + H2O --> L-cysteinylglycine + L-glutamate (according to UniProt)
an S-substituted glutathione + H2O --> an S-substitued L-cysteinylglycine + L-glutamate, however, all evidence suggests this is a misannotation PubMed
Protein family
Structure
2NLZ (PDB) (from B. halodurans, 38% identity)
Additional information
The gene is annotated in KEGG as an ortholog of gamma-glutamyltranspeptidase EC 2.3.2.2, This enzyme is necessary to utilize glutathione (GSH) as the sulfur source. No EC annotation for this gene is available in Swiss-Prot/MetaCyc. It has been shown by Minami et al. (PubMed) that ywrD mutant grows well on minimal media supplied with GSH as the sole sulfur source. In addition, His-tag purified ywrD cannot hydrolyze GSH. PubMed
Expression and Regulation
Operons
Biological materials
Mutant
MGNA-B653 (ywrD::erm), available at the NBRP B. subtilis, Japan
References
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Time of last update: 2025-01-14 23:39:02
Author of last update: Melvin.boenninger