yhcW

168

putative glycerol-3-phosphatase

Locus: BSU_09240

Molecular weight: 24.53 kDa

Isoelectric point: 4.63

Protein length: 220 aa

Gene length: 663 bp

Function: unknown

Product: unknown

Essential: no

Synonyms: yhcW

Genomic Context

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Categories containing this gene/protein

Groups of genes, Poorly characterized/ putative enzymes

List of homologs in different organisms, belongs to COG0637 (Galperin et al., 2021)

Gene

Coordinates: 997,724  998,386

The protein

Catalyzed reaction/ biological activity

glycerol-3-phosphate --> glycerol + inorganic phosphate PubMed

Protein family

HAD superfamily (according to UniProt)
CbbY/CbbZ/Gph/YieH family (with PgcM, according to UniProt)

Structure

4UAR (PDB) (from Rhodobacter Sphaeroides, 32% identity)
P54607 (AlphaFold)

Paralogous protein(s)

PgcM, (31%)

Expression and Regulation

Operons

Genes: yhcW-yhcX

Description: PubMed

Biological materials

Mutant

MGNA-A660 (yhcW::erm), available at the NBRP B. subtilis, Japan
BKE09240 (yhcW::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACTGGGTACAACTCCTTTA,  downstream forward: _UP4_CAAAACTAGAAAGGAGCGAG
BKK09240 (yhcW::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACTGGGTACAACTCCTTTA,  downstream forward: _UP4_CAAAACTAGAAAGGAGCGAG

References

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