ydfD

168

similar to transcriptional regulator (MocR/ GabR family)

Locus: BSU_05370

Molecular weight: 54.07 kDa

Isoelectric point: 9.1

Protein length: 482 aa

Gene length: 1449 bp

Function: unknown

Product: unknown

Essential: no

Synonyms: ydfD

Genomic Context

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List of homologs in different organisms, belongs to COG1167 (Galperin et al., 2021)

Gene

Coordinates: 583,589 → 585,037

The protein

Protein family

• class-I pyridoxal-phosphate-dependent aminotransferase family (according to UniProt)
• MocR/ GabR family PubMed

Domains

• N-terminal DNA-binding helix-turn-helix motif; C-terminal domain is homologous to PLP-binding large domain of aminotransferases.
• HTH gntR-type domain (aa 12-80) (according to UniProt)

Cofactors

• PLP

Structure

• 1WST (PDB) (aminotransferase from Thermococcus profundus, 30% identity)
• P96681 (AlphaFold)

Paralogous protein(s)

• YisV

Biological materials

Mutant

• MGNA-C144 (ydfD::erm), available at the NBRP B. subtilis, Japan
• BKE05370 (ΔydfD::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAGAATCGGTCTCCTTAA,  downstream forward: _UP4_TAACTTTTATATCCTTAAAT
• BKK05370 (ΔydfD::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAGAATCGGTCTCCTTAA,  downstream forward: _UP4_TAACTTTTATATCCTTAAAT

References

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